General

We are currently using a proprietary model to predict whether or not a probe will be difficult to detect by Illumina sequencing. The inputs to this model include GC content, global complexity (measured as the number of distinct kmers contained in a probe sequence), local complexity (measured as the number of distinct kmers within a limited region of a sequence), and the presence of homopolymers.

Generally, probes with GC content > 85%, homopolymers longer than 20 nt, or tandem repeats of di- or tri- nucleotides longer than 30 nt will be difficult to sequence.

We do not make RNA probes. However, our DNA probes can be used to enrich targets from cDNA libraries made from RNA.

Yes, although it is recommended you consult with your local Twist Account Manager for more information. We can design panels using 80bp, 100bp, and 120bp probe lengths. 

There are no size limitations on custom panel designs.

Yes, please contact customersupport@twistbioscience or your Account Manager for more information.  

Twist's probes for target enrichment are double-stranded DNA that target both strands for improved sensitivity. They can also be used to enrich targets from cDNA libraries made from RNA.

We are currently using a proprietary model to predict whether or not a probe will be difficult to detect by Illumina sequencing. The inputs to this model include GC content, global complexity (measured as the number of distinct kmers contained in a probe sequence), local complexity (measured as the number of distinct kmers within a limited region of a sequence), and the presence of homopolymers.

Generally, probes with GC content > 85%, homopolymers longer than 20 nt, or tandem repeats of di- or tri- nucleotides longer than 30 nt will be difficult to sequence.

We do not make RNA probes. However, our DNA probes can be used to enrich targets from cDNA libraries made from RNA.

Yes, although it is recommended you consult with your local Twist Account Manager for more information. We can design panels using 80bp, 100bp, and 120bp probe lengths. 

There are no size limitations on custom panel designs.

Yes, please contact customersupport@twistbioscience or your Account Manager for more information.  

Twist's probes for target enrichment are double-stranded DNA that target both strands for improved sensitivity. They can also be used to enrich targets from cDNA libraries made from RNA.