Placing An Order

To resume editing a draft, follow these steps: 

1. Log-in to your account
2. On the left hand side of the screen, there will be a section called Orders & Drafts. Click on Drafts. 
3. Click on the name of the Draft you want to edit. 
4. You will now be able to proceed with making changes to the Draft. 

*If the draft is already in the checkout phase, you will be able to go back and edit by clicking on the “Edit Order” button. 

Shipping Addresses can be added during the checkout process. However, if you need to update the address for your account without placing an order, you can follow these steps: 

1. Log-in to your account

2. On the left hand side of the page, click on Shipping Addresses (under Account)

3. There will be the option to add a new address by clicking on the “New address” button. 

4. You will be prompted to enter your shipping information.

The easiest and fastest way to receive a quote is to create an account on our eCommerce site. As soon as your account is set up, you can create a new draft project for Synthetic Biology (Gene Fragments/Clonal Genes/Oligo Pools) or Next Generation Sequencing products (DNA Library Preparation and Enrichment Panels).

Payment methods can be added when you are checking out for your order. If you need to add a payment prior to placing an order, you can follow these steps:

1. Log-in to your account

2. On the left hand side of the page, go to Account

3 Click on Payment Method

4. There will be the option to add a credit card or add a PO.

     a. Credit Card: customer is paying via a credit card

     b. PO: customer is paying via a PO and will be invoiced once their order ships

**Please note uploading the PO does not place the order (it only stores the PO in your account). You will still need to checkout to place the order. Once you checkout, a member of our Support team will review the order and move it into production. 

**For questions around BPOs, please contact our Customer Support team at customersupport@twistbioscience.com. 

The Twist Codon Optimizer Tool randomly assigns synonymous codons based on the selected organism's codon preferences. Here is a list of considerations during optimization:

• We assign more common codons more frequently than less common ones
• We avoid using rare codons (those with a codon frequency of <8%)
• We ensure sequences do not have strong hairpins (∆G <-8) in the first 48 base pairs of resulting sequences
• We check both strands to ensure they don't contain the enzyme cut sites you asked us to avoid creating
• We avoid introduction of promoter sequences internal to expression sequence by avoiding the creation of strong sigma70 binding sites
• We avoid sequences that create strong ribosome binding sites (GGAGG and TAAGGAG)
• We avoid sequences that create terminator sequences (TTTTT or AAAAA) Since the most preferred codon is not always assigned, you should not expect to see the same output after each optimization attempt (no 1:1 codon to amino acid assignment).
• You should expect to see a different optimized sequence if you optimize the same sequence multiple times.

Please note that optimal expression cannot be guaranteed for any sequence that goes through Twist optimization as there are many factors that go into quality expression, some of which are not well understood.

Additional factors, such as mRNA stability, protein maturation, translational speed, chaperone expression, promoter design, expression vector etc. also influence expression.

To resume editing a draft, follow these steps: 

1. Log-in to your account
2. On the left hand side of the screen, there will be a section called Orders & Drafts. Click on Drafts. 
3. Click on the name of the Draft you want to edit. 
4. You will now be able to proceed with making changes to the Draft. 

*If the draft is already in the checkout phase, you will be able to go back and edit by clicking on the “Edit Order” button. 

Shipping Addresses can be added during the checkout process. However, if you need to update the address for your account without placing an order, you can follow these steps: 

1. Log-in to your account

2. On the left hand side of the page, click on Shipping Addresses (under Account)

3. There will be the option to add a new address by clicking on the “New address” button. 

4. You will be prompted to enter your shipping information.

The easiest and fastest way to receive a quote is to create an account on our eCommerce site. As soon as your account is set up, you can create a new draft project for Synthetic Biology (Gene Fragments/Clonal Genes/Oligo Pools) or Next Generation Sequencing products (DNA Library Preparation and Enrichment Panels).

Payment methods can be added when you are checking out for your order. If you need to add a payment prior to placing an order, you can follow these steps:

1. Log-in to your account

2. On the left hand side of the page, go to Account

3 Click on Payment Method

4. There will be the option to add a credit card or add a PO.

     a. Credit Card: customer is paying via a credit card

     b. PO: customer is paying via a PO and will be invoiced once their order ships

**Please note uploading the PO does not place the order (it only stores the PO in your account). You will still need to checkout to place the order. Once you checkout, a member of our Support team will review the order and move it into production. 

**For questions around BPOs, please contact our Customer Support team at customersupport@twistbioscience.com. 

The Twist Codon Optimizer Tool randomly assigns synonymous codons based on the selected organism's codon preferences. Here is a list of considerations during optimization:

• We assign more common codons more frequently than less common ones
• We avoid using rare codons (those with a codon frequency of <8%)
• We ensure sequences do not have strong hairpins (∆G <-8) in the first 48 base pairs of resulting sequences
• We check both strands to ensure they don't contain the enzyme cut sites you asked us to avoid creating
• We avoid introduction of promoter sequences internal to expression sequence by avoiding the creation of strong sigma70 binding sites
• We avoid sequences that create strong ribosome binding sites (GGAGG and TAAGGAG)
• We avoid sequences that create terminator sequences (TTTTT or AAAAA) Since the most preferred codon is not always assigned, you should not expect to see the same output after each optimization attempt (no 1:1 codon to amino acid assignment).
• You should expect to see a different optimized sequence if you optimize the same sequence multiple times.

Please note that optimal expression cannot be guaranteed for any sequence that goes through Twist optimization as there are many factors that go into quality expression, some of which are not well understood.

Additional factors, such as mRNA stability, protein maturation, translational speed, chaperone expression, promoter design, expression vector etc. also influence expression.