Delayed flowering and prolonged juvenile phase, two major hindrances to conventional cassava breeding could be overcome by transiently overexpressing or silencing flowering related genes. Here, we used the East African Cassava Mosaic Virus Kenya (EACMV-K) to develop a transient gene delivery system with an overall goal of manipulating flowering. The deconstructed forms of the DNA A of EACMV-K were used to deliver Arabidopsis thaliana and Mahihot esculenta (cassava) Flowering Locus T genes ( AtFT and MeFT1respectively) into Nicotiana benthamiana, N. tabacum and cassava. The deconstructed viruses were also used to deliver small interfering RNA (siRNA) targeting M. esculenta Terminal Flower 1 (MeTFL) in cassava. Southern blot analyses demonstrated successful gene delivery and replication in N. benthamiana and N. tabacum, with minimal replicative forms in cassava. Quantitative PCR analysis of MeTFL indicated that gene silencing occurred. The days to flowering (DTF) were observed to be less in the AtFT and MeFT1 infiltrations relative to the control (P