The combination of genetic circuits is typically thought of as a futile endeavor due to the sensitivity for them to fail when additional genetic elements are added. The construction of a combined genetic toggle-switch repressilator is mathematically feasible and within the bound of construction using available bioengineering techniques. Such a genetic circuit would display behavior analogous to that of an AC - DC circuit, which presents interesting possible applications in synthetic biology. Through the use of PCR, Gibson Assembly, T4 ligation, and short oligonucleotide synthesis, a repressilator containing a hybrid toggling promoter was constructed. Additionally, three reporter plasmids to allow for visualization of oscillations were constructed. From preliminary testing utilizing Tecan growth assays and microfluidic chip experiments, the circuit is shown to be both a bistable toggle and an oscillatory system, with possible faster oscillation than seen in the original repressilator designed. Additionally, due to the toggling behavior, clamping of the circuit is not required, permitting easier use in research environments, yet further study is required. Main Content Download PDF to ViewView Larger For improved accessibility of PDF content, download the file [/content/qt77z066c7/qt77z066c7.pdf?t=sfl1s9] to your device.