DNA is considered as an ideal supramolecular material for information storage with high storage density and long-term stability. Enzymes, as green and sustainable tools, offer several unique advantages for DNA-based information storage. These advantages include low cost and reduced generation of hazardous wastes during DNA synthesis, as well as the improvements in data reading speed and data recovery accuracy. Moreover, enzymes could achieve scalable data steganography. In this review, we introduced the exciting application strategies of enzymatic tools in each step of DNA information storage (writing, storing, retrieval and reading). We further address the challenges and opportunities associated with enzymatic tools for DNA information storage, aiming at developing new techniques to overcome these obstacles.