Enterobacter sp. YSU is one of multiple metal-resistant bacteria isolated from East Fork Poplar Creek near a nuclear plant in Oak Ridge, Tennessee. This bacteria has a previously-detailed resistance to selenite toxicity, with the genes and proteins responsible yet to be fully elucidated. It can grow in the presence of 1 M sodium selenite in a rich LB broth and reduces the selenite to red selenium precipitates in culture. This study examines possible overexpression of ompA, ompX, dedA, and gutS genes in Enterobacter sp. YSU in the presence of selenite. The four genes of interest were identified by previous studies. Overnight cell cultures of the Enterobacter sp. YSU strain were grown in a rich Luria-Bertani broth for 5 hours. One culture had 1 M sodium selenite added, the other water for control. Absorbance readings were taken every 30 minutes to monitor cell growth and RNA was extracted during log phase, 2.5 hours into the experiment, for an RT-PCR procedure. This RNA was subsequently converted to cDNA and amplified with primers for the four genes, then ran on 2% agarose gels. Both ompX and dedA displayed increased expression when grown with selenite but ompA and gutS had no difference in expression between the selenite culture and the control. It is possible that ompA had no overexpression because both of the omp proteins are hypothesized to form a single protein. Consistent low expression of gutS may be due to high amounts of this porin protein damaging the YSU cell membrane, due to selenite-induced apoptosis. Further work through qPCR could further elucidate the expression levels of these four genes in response to selenite.