A two-plasmid CRISPRi system for Clostridioides difficile that does not require antibiotic maintenance was developed. pJAK184.tetR.PT5-3.dCas9 contains an optimized tetracycline-inducible dCas9 for chromosomal insertion. pJC.15A.sgRNA.TA encodes a toxin-antitoxin system for stable maintenance, and mCherry, which is exchangeable for a customized sgRNA. We demonstrate the knockdown of the essential gene walA.