Shrimp farming that has experienced rapid growth globally faces significant economic challenges due to frequent disease outbreaks. In the absence of a fully developed immune system, immune enhancement shows the only option for reducing disease susceptibility. Prebiotics foster the growth of beneficial gut bacteria and play a key role in gut metabolism contributing to the immune enhancement. In the present study, inulin coated feed was used as a candidate prebiotic to study the effect on the growth, disease resistance, immune gene expression, and gut microbial diversity of Litopenaeus vannamei. Three concentrations of inulin was used coated to the normal basal feed (T1: 5 g/kg, T2: 10 g/kg, and T3: 20 g/kg) and compared with the control group (with no inulin) during a course of 30 days. Relatively, higher growth was observed in all the experimental groups. Significant (P