Transgenic markers and tools have revolutionised how we study cells and developing organisms. Some of the elements needed to construct those tools are universally applicable (e.g. fluorescent proteins), while others are species-specific (e.g.cis-regulatory elements driving transcription). Membrane localisation signals targeting proteins to the plasma membrane have been identified in several model organisms. Unfortunately, these do not work equally well in different species, developmental stages and cell types. To generate a toolkit of membrane localisation tags that can be screened rapidly in diverse species, we collected 11 membrane localisation tags -known to localise proteins to the plasma membrane through different mechanisms- and fused them to the red fluorescent protein mScarlet3. Each tagged construct was placed downstream of a T7 promoter, allowing thein vitroproduction of tagged reporter mRNAs, which can be readily delivered to embryos or cell types of interest. The tags in this kit include signals for trafficking to the plasma membrane through the exocytic pathway with a transmembrane domain (SP-CD8tm) or a GPI anchor (SP-GPI), lipid-binding domains (PH, LactC2), signals for prenylation, palmitoylation and myristoylation (H-Ras, K-Ras, K-Ras-6R, GAP43, Lyn, Src64B), and a domain that targets proteins to the plasma membrane independently of exocytosis or lipid modification (RitC). We demonstrate the use of this toolkit in early embryos of the crustaceanParhyale hawaiensisand primary polyps of the hydrozoanClytia hemisphaerica.