The Importance Of Coverage Uniformity Over On-Target Rate For Efficient Targeted NGS

Coverage uniformity is a rarely discussed quality metric. We show it to be important for downstream experimental costs and efficacy.

View the White Paper

Download the White Paper

In exome sequencing the design and production of target enrichment probe panels have an inherent effect on capture efficiency. In this paper, we use ranges of on-target rate and uniformity typical of commercial exome kits to mathematically model the relative impacts of both metrics on overall experimental efficiency. We demonstrate that, though most commercial probe panels cite only on-target rate in their specifications, uniformity has a more significant contribution to the efficiency and oversampling requirements in targeted sequencing experiments.

Covered in this White Paper

In depth analysis of uniformity metrics calculation

Impacts of uniformity optimization on on-target rate

Share your details to Get the White Paper

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

The Importance Of Coverage Uniformity Over On-Target Rate For Efficient Targeted NGS

Related Resources

Highly Sensitive Methylation Detection Using Enzymatic Methyl-seq and Twist Target Enrichment

Twist Targeted Methylation Sequencing Protocol

NGS Methylation Detection System