Publications
Reaction Chemistry & EngineeringApr 2023 DOI:
10.1039/d3re00002h

Directed evolution of Rhodotorula gracilisd-amino acid oxidase using single-cell hydrogel encapsulation and ultrahigh-throughput screening

Küng, Christoph; Vanella, Rosario; Nash, Michael A.
Product Used
Genes
Abstract
A high-throughput screening platform uses peroxidase-mediated proximity labeling with fluorescent hydrogels. A yeast displayed enzyme variant library was screened using a reaction cascade. Enhancement of enzyme Km is observed for displayed enzymes.
Product Used
Genes

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