Plant scientists must develop new strategies to enable crops to adapt to biotic and abiotic stresses that reduce yield. The polyamines putrescine, spermidine, spermine, and thermospermine are important metabolites that mediate changes in development and stress responses and thus are potential targets for genetic engineering to modify crop metabolism. Our immediate goal is to define where the proteins associated with putrescine synthesis and transport are localized. Agmatine iminohydrolase (AIH) has five alternative splice forms that are translated into four different proteins. In prior work, all these splice forms of AIH were localized to the endoplasmic reticulum (ER) except AIH.4 was localized in the chloroplast. Here I demonstrated that the N-terminal sequence fusion of AIH.4-GFP was localized to the chloroplast. bidirectional amino acid transporter1 (BAT1) and organo-cation transporter 1 (OCT1) are two polyamine transporters that have only been partially characterized. BAT1 has two alternative splices, BAT1.1 and BAT1.2, localized to the ER and the chloroplast. Here I demonstrated that the N-terminal sequence of the membrane transporter BAT1.1 was sufficient to traffic N-Term-BAT1.1-GFP to the ER. Finally, I demonstrated that OCT1 was localized to the ER by co-expression of OCT1-GFP with the ER-mCherry molecular marker. This finding is especially significant, as OCT1 is a known exporter and its localization to the ER points to the significance of ER-directed secretion of metabolites