Many bacteria sense their surrounding environment and move accordingly via chemoreceptor proteins in a process known as chemotaxis. Members of the Dickeya genus, which cause disease on numerous crops and ornamental plants, have notoriously more predicted methyl-accepting chemotaxis proteins (MCPs) than other closely related bacteria. However, the functions and signals of many of the putative MCPs remain unknown. Interestingly, long untranslated regions exist upstream of the coding regions of many of the MCPs in Dickeya dadantii 3937. RNA-Seq and RT-qPCR showed that these regions are transcribed in planta. Transcription start sites (TSSs) were identified using Cappable-seq and aligned well with the areas being transcribed. Using in silico methods we also identified the potential promoters and terminators of these transcription units, which we termed Regions of Activity (ROAs). Mutants lacking the ROAs, or the MCP genes, were constructed and tested for their ability to swim and swarm in vitro, their chemotaxis capability towards multiple compounds, and their virulence in potato tubers. Differences in motility, chemotaxis, and virulence were found between some of the mutants and the wild-type. Interestingly, the deletion of three ROA-MCP pairs caused a significant increase in tuber lesion size compared to the wild-type. One MCP mutant showed a decreased attraction to jasmonic acid. Our results provide new insight into the sensing and signaling mechanisms used by Dickeya and may provide targets for disease control.