CMV driven expression vector for transient expression in mammalian cells. This vector contains an ampicillin resistance cassette for growth and maintenance in E.coli. This vector has a reduced overall size due to the absence of a mammalian selection marker. Transcriptional termination is via a SV40 poly-adenylation signal 3’ of the multiple cloning site.

A CMV driven expression vector for high level transient expression in mammalian cells which contains an ampicillin resistance cassette for growth and maintenance in E.coli. The OriP drives plasmid replication in EBNA transformed HEK-293 cells facilitating higher levels of protein expression. Transcriptional termination is performed via a SV40 poly-adenylation signal 3’ of the multiple cloning site.

T7 RNA polymerase driven transcription vector for expression in E. coli. The vector, which lacks the ribosome binding site and ATG start codon, is designed for protein expression from translation signals carried by the cloned DNA. Vector features: C-terminal His•Tag® sequence lac repressor / lac operator to inhibit transcription in E. coli. Expression can be induced by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage.

T7 RNA polymerase driven transcription vector for expression in E. coli. The vector, which lacks the ribosome binding site and ATG start codon, is designed for protein expression from translation signals carried by the cloned DNA. Vector features: C-terminal His•Tag® sequence lac repressor / lac operator to inhibit transcription in E. coli. Expression can be induced by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage.

Transcription/translation vector for protein expression in E. coli. The vector, which contains the ribosome binding site and ATG start codon, is designed for protein expression from translation signals carried by the cloned DNA. Translation reading frame relative to the GGATCC BamH I cloning site is GGA. Vector features: N-terminal and optional C-terminal His•Tag® sequences Internal T7•Tag® sequence Thrombin cleavage site lac repressor / lac operator to inhibit transcription in E. coli. Expression can be induced by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage.

Transcription/translation vector for protein expression in E. coli. The vector, which contains the ribosome binding site and ATG start codon, is designed for protein expression from translation signals carried by the cloned DNA. Translation reading frame relative to the GGATCC BamH I cloning site is GAT Vector features: N-terminal S•Tag™ sequence C-terminal His•Tag® sequence Thrombin cleavage site lac repressor / lac operator to inhibit transcription in E. coli. Expression can be induced by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage.

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pCCL lentivirus backbone. The SFFV promoter is present to drive transgene expression. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix.

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pCCL lentivirus backbone. The SFFV promoter is present to drive transgene expression. The plasmid also contains a gene allowing the antibiotic puromycin whose expression is driven by the SSFV promoter via a EMCV IRES. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix.

CMV driven expression vector for transient expression in mammalian cells. In addition to the CMV promoter this vector contains a beta globin intron that has demonstrated ability to enhance gene expression. This vector contains an ampicillin resistance cassette for growth and maintenance in E.coli. This vector has a reduced overall size due to the absence of a mammalian selection marker. Transcriptional termination is via a SV40 poly-adenylation signal 3’ of the multiple cloning site.

Human Elongation Factor Alpha Promoter (EF1 Alpha) driven medium level transient mammalian expression vector. This vector contains an ampicillin resistance cassette for growth and maintenance in E.coli. This vector has a reduced overall size due to the absence of a mammalian selection marker. Transcriptional termination is via a SV40 poly-adenylation signal 3’ of the multiple cloning site.

Human Elongation Factor Alpha Promoter (EF1 Alpha) driven medium level mammalian expression vector for generation of stable cell lines via Puromycin selection. This vector contains a ampicillin resistance cassette for growth and maintenance in E.coli. Transcriptional termination is via a SV40 poly-adenylation signal 3’ of the multiple cloning site.

A CMV driven expression vector for high level transient expression in mammalian cells which contains an ampicillin resistance cassette for growth and maintenance in E.coli. This vector contains a Hygromycin mammalian selection marker for the generation of stable mammalian clones. Transcriptional termination is performed via a SV40 poly-adenylation signal 3’ of the multiple cloning site.

A CMV driven expression vector for high level transient expression in mammalian cells which contains an ampicillin resistance cassette for growth and maintenance in E.coli. This vector contains a Puromycin mammalian selection marker for the generation of stable mammalian clones. Transcriptional termination is performed via a SV40 poly-adenylation signal 3’ of the multiple cloning site.

CMV driven expression vector for transient expression in mammalian cells designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted and adherent cells for transient protein expression. The vector can also be used as a G-418-selectable expression plasmid for engineering stable cell lines. In addition to the CMV promoter this vector contains WPRE that has demonstrated the ability to enhance transgene expression.

pTwist CMV hIgL2 is an expression vector that contains the constant region of human lambda 2 light chain. When co-transfected into mammalian cells with the heavy chain variable region cloned into pTwist hIgG1/2/4 S228P, the antibody of interest is produced.

pTwist CMV hIgG2 is an expression vector that contains the constant region of human IgG2 isotype, which has minimal effector function. When co-transfected into mammalian cells with the light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced.

pTwist CMV hIgG1-Fc is an expression vector that contains a G4S linker fused to the CH2 and CH3 region of human IgG1 isotype.

pTwist CMV hIgK is an expression vector that contains the constant region of human kappa light chain. When co-transfected into mammalian cells with the heavy chain variable region cloned into pTwist hIgG1/2/4 S228P, the antibody of interest is produced.

pTwist CMV hIgG1 is an expression vector that contains the constant region of human IgG1 isotype. When co-transfected into mammalian cells with the light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced.

CMV driven expression vector for transient expression in mammalian cells designed to deliver exceptionally high levels of transgene expression. This vector can be used in suspension-adapted and adherent cells for transient protein expression and can also be used as a G-418-selectable expression plasmid for stable cell line engineering. In addition to the CMV promoter, this vector contains a beta globin intron and a WPRE which have demonstrated the ability to enhance transgene expression. For IgG Expression, this vector is available for full-length or single-chain expression wherein the desired Fc regions differ from the available IgG vector offering. This allows for customizable Fc sequence regions such as mouse or rabbit Fc regions.

pTwist CMV hIgG4 S228P is an expression vector that contains the constant region of human IgG4 S228P isotype. The S228P mutation reduces Fab-arm exchange by stabilizing the disulfides in the core-hinge of the IgG4 molecules. When co-transfected into mammalian cells with the light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced.

pTwist CMV hIgG2-Fc is an expression vector that contains a G4S linker fused to the CH2 and CH3 region of human IgG2 isotype, which has minimal effector function.

T7 RNA polymerase driven transcription vector for expression in E. coli. The vector, which lacks ATG start codon and stop codon, is designed for protein expression from translation signals carried by the cloned DNA. Vector features: lac repressor / lac operator to inhibit transcription in E. coli. Expression can be induced by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage. Insertion sites: T7-lacO-RBS - Does not contain ATG and stop codon. These should be added to the insert during ordering. T7-lacO (no RBS) - Does not contain RBS, ATG and stop codon. These should be added to the insert during ordering. Custom promoter-RBS - Does not contain a promoter, RBS, ATG and stop codon. These should be added to the insert during ordering.

T7 RNA polymerase driven transcription vector for expression in E. coli. The vector, which lacks ATG start codon and stop codon, is designed for protein expression from translation signals carried by the cloned DNA. Vector features: lac repressor / lac operator to inhibit transcription in E. coli. Expression can be induced by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG) Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage. Insertion sites: T7-lacO-RBS - Does not contain ATG and stop codon. These should be added to the insert during ordering. T7-lacO (no RBS) - Does not contain RBS, ATG and stop codon. These should be added to the insert during ordering. Custom promoter-RBS - Does not contain a promoter, RBS, ATG and stop codon. These should be added to the insert during ordering.

T7 RNA polymerase driven transcription vector for expression in E. coli. The vector, which lacks ATG start codon and stop codon, is designed for protein expression from translation signals carried by the cloned DNA. Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage. This vector can be used for IVT with the proper addition of linearization enzymes added to the 3' end of the 3' UTR Insertion sites: T7-RBS - Does not contain ATG and stop codon. These should be added to the insert during ordering. T7 (no RBS) - Does not contain RBS, ATG and stop codon. These should be added to the insert during ordering. Custom promoter-RBS - Does not contain a promoter, RBS, ATG and stop codon. These should be added to the insert during ordering.

T7 RNA polymerase driven transcription vector for expression in E. coli. The vector, which lacks ATG start codon and stop codon, is designed for protein expression from translation signals carried by the cloned DNA. Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage. This vector can be used for IVT with the proper addition of linearization enzymes added to the 3' end of the 3' UTR Insertion sites: T7-RBS - Does not contain ATG and stop codon. These should be added to the insert during ordering. T7 (no RBS) - Does not contain RBS, ATG and stop codon. These should be added to the insert during ordering. Custom promoter-RBS - Does not contain a promoter, RBS, ATG and stop codon. These should be added to the insert during ordering.

Twist’s version of pcDNA3.1(+)™. CMV driven expression vector for transient expression in mammalian cells designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted and adherent cells for transient protein expression. T7 promoter, Bovine Growth Hormone (BGH) polyadenylation signal and termination signal are also present to allow for in vitro transcription applications with enhanced mRNA stability. Insertion sites: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by BamHI and NotI recognition sites for restriction enzyme digestion to separate the insert from the backbone.

Twist’s version of pcDNA3.4(+)™. CMV driven expression vector for transient expression in mammalian cells designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted and adherent cells for transient protein expression. In addition to the CMV promoter this vector contains WPRE that has demonstrated the ability to enhance transgene expression. HSV TK polyA signal and termination signal are present for enhanced mRNA stability. Insertion sites: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by Esp3I and EcoRV recognition sites for restriction enzyme digestion to separate the insert from the backbone.

T7 RNA polymerase driven transcription vector for expression in E. coli. The vector, which lacks the ribosome binding site and ATG start codon, is designed for protein expression from translation signals carried by the cloned DNA. Vector features: C-terminal His•Tag® sequence. Production of virions containing single-stranded DNA corresponding to the coding strand upon co-transfection with helper phage. Insertion sites: pET-23(+)_default - Does not contain RBS and ATG. These should be added to the insert during orderingadds on AAALE peptide linker sequence between gene of interest and C-terminal His•Tag® sequence

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pLKO lentivirus backbone. The CMV promoter is present to drive transgene expression. The plasmid also contains a gene allowing the antibiotic blasticidin selection whose expression is driven by human phosphoglycerate kinase 1 (PGK1) promoter. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix. Insertion site: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by NheI and EcoRV recognition sites for restriction enzyme digestion to separate the insert from the backbone.

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pLKO lentivirus backbone. The CMV promoter is present to drive transgene expression. The plasmid also contains a gene allowing the antibiotic Puromycin selection whose expression is driven by human phosphoglycerate kinase 1 (PGK1) promoter. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix. Insertion site: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by NheI and EcoRV recognition sites for restriction enzyme digestion to separate the insert from the backbone.

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pLKO lentivirus backbone. The tight TRE promoter is present to drive low level transgene expression. The plasmid also contains a gene allowing the antibiotic Puromycin whose expression is driven by human phosphoglycerate kinase 1 (PGK1) promoter downstream of the gene of interest. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix. Insertion site: No_tag - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. 6xHis_tag - Does not contain kozak sequence, start codon, or stop codon. Contains a C-terminal 6xHis•Tag®

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pLKO lentivirus backbone. The Chicken-β-actin (CAG) promoter is present to drive transgene expression. The plasmid also contains a gene allowing the antibiotic puromycin selection whose expression is driven by human phosphoglycerate kinase 1 (PGK1) promoter. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix. Insertion site: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by BstXI and FseI recognition sites for restriction enzyme digestion to separate the insert from the backbone.

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pLKO lentivirus backbone. The Human Elongation Factor Alpha Promoter (EF1α) is present to drive transgene expression. The gene expression is also regulated by WPRE on the 3'-end of the gene. The plasmid also contain a CMV-driven reporter EGFP linked to Puromycin resistance with a T2A peptide-linker. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix. Insertion site: WPRE_EGFP_T2A_Puro - Does not contain kozak sequences, start codon or stop codon. These should be added to the insert during ordering. T2A_Puro - Does not contain kozak sequences, start codon, WPRE and the reporter EGFP. These should be added to the insert during order. This insertion site is right upstream of the T2A. Stop codon should not be added to the insert or the puromycin expression will be affected.

A lentivirus expression plasmid that can be used for the stable integration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pLKO lentivirus backbone. The SDFV promoter is present to drive transgene expression. The plasmid also contains a gene allowing the antibiotic puromycin selection whose expression is driven by human phosphoglycerate kinase 1 (PGK1) promoter. This is a third generation lenti virus that can be packaged using either second or third generation packaging mix. Insertion site: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by NheI and XbaI recognition sites for restriction enzyme digestion to separate the insert from the backbone.

Chicken-β-actin (CAG promoter) driven expression vector, which allows for long term and stable protein expression in a large variety of mammalian cell line including stem cells. It can be used in suspension-adapted and adherent cells for transient protein expression. In addition to the CAG promoter, this vector contains chimeric intron upstream and WPRE downstream of the gene of interest for enhanced transgene expression.HSV TK polyA signal and termination signal are present for enhanced mRNA stability. Insertion sites: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by BstXI and FseI recognition sites for restriction enzyme digestion to separate the insert from the backbone.

CMV expression vector for transient expression in mammalian cells designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted and adherent cells for transient protein expression. In addition to the CMV promoter, this vector containsβ-globin intron upstream and WPRE downstream of the gene of interest for enhanced transgene expression.HSV TK polyA signal and termination signal are present for enhanced mRNA stability. Insertion sites: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by BstXI and FseI recognition sites for restriction enzyme digestion to separate the insert from the backbone.

Human Elongation Factor Alpha Promoter (EF1α) driven medium level mammalian expression vector for generation of stable cell lines via Neomycin selection. This vector contains a ampicillin resistance cassette for growth and maintenance in E.coli. Transcriptional termination is via a HSV TK polyA signaldownstream of the multiple cloning site. Insertion sites: Default - Does not contain kozak sequence, start codon, or stop codon. These should be added to the insert during ordering. Default insertion site is flanked by NheI and XbaI recognition sites for restriction enzyme digestion to separate the insert from the backbone.

Twist's in vitro transcription (IVT) vector based on the pTwist Kan High Copy v2 backbone. The vector features the human β-globin (hBG) 5'UTR upstream and hBG 3'UTR downstream the gene of interest. A 50A polyA tail is included at the 3' end of the 3' UTR. Plasmid linearization is enabled by BspQI, which exposes the polyA tail directly. Multiple blunt enzyme cut sites are available downstream of the polyA tail to allow for flexibility. Insertion site: Default - Does not contain kozak sequences, start codon or stop codon. These should be added to the insert during ordering

pTwist CMV rbIgk2 is an expression vector that contains the Rabbit Igk2 light constant region. When co-transfected into mammalian cells with a heavy chain variable region cloned into pTwist CMV rbIgG1, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV rbIgk1 is an expression vector that contains the Rabbit Igk1 light constant region. Note that the Igk1 light chain constant region contains an unpaired Cys that typically pairs with an unpaired Cys in the light chain variable region. Ensure the inserted light chain variable region contains the unpaired Cys in the appropriate region. When co-transfected into mammalian cells with a heavy chain variable region cloned into pTwist CMV rbIgG1, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV rbIgG1 is an expression vector that contains the Rabbit IgG heavy constant region. When co-transfected into mammalian cells with a light chain variable region cloned into pTwist CMV rbIgk1 or pTwist CMV rbIgk2, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV mIgL2 is an expression vector that contains the Mouse Ig Lambda 2 constant region. When co-transfected into mammalian cells with the heavy chain variable region cloned into pTwist CMV mIgG2a, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV mIgL1 is an expression vector that contains the Mouse Ig Lambda 1 constant region. When co-transfected into mammalian cells with the heavy chain variable region cloned into pTwist CMV mIgG2a, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV mIgk is an expression vector that contains the Mouse Ig kappa constant region. When co-transfected into mammalian cells with the heavy chain variable region cloned into pTwist CMV mIgG2a, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV mIgG2a (truncated) is an expression vector that contains the Mouse truncated IgG2a sequence. This vector can be used for VHH expression or other protein expression and should NOT be co-transfected with a light chain expression vector. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV mIgG2a is an expression vector that contains the Mouse IgG2a heavy constant region (mIgG2a). When co-transfected into mammalian cells with the light chain variable region cloned into pTwist CMV mIgK or mIgL2 or mIgL2, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV bispecific hIgG1 (knob) is an expression vector that contains the Human IgG1 bispecific constant with knob mutations. When co-transfected into mammalian cells with a heavy chain variable region cloned into pTwist CMV bispecific hIgG1 (hole) and a light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV bispecific hIgG1 (hole) is an expression vector that contains the Human IgG1 bispecific full constant region with hole mutations. When co-transfected into mammalian cells with a heavy chain variable region cloned into pTwist CMV bispecific hIgG1 (knob) and a light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV hIgG1_LS is an expression vector that contains the constant region of human IgG1 isotype with M428L/N434S (LS) mutation. M428L/N434S are half-life extending mutations in the Fc region of human IgG1 antibodies. When co-transfected into mammalian cells with the light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV hIgG1 (LALA+PG+YTE) is an expression vector that contains the constant region of human IgG1 isotype with L234A/L235A+P329G+M252Y/S254T/T256E mutations to help with effector function silencing. When co-transfected into mammalian cells with the light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.

pTwist CMV bispecific hIgG1 (stump hole) is an expression vector that contains a truncated Human IgG1 bispecific constant region with hole mutations. When co-transfected into mammalian cells with a heavy chain variable region cloned into pTwist CMV bispecific hIgG1 (knob) and a light chain variable region cloned into pTwist hIgK or hIgL2, the antibody of interest is produced in monovalent binding format with the binding arm on the knob side. Insertion site does not contain kozak or signaling peptide sequences and should be included during ordering.