Combinatorial Assembly Libraries
Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR), chimeric antigen receptor (CAR), and multispecific VHH libraries all through our combinatorial assembly libraries.
Combinatorial Assembly Libraries utilize seamless assembly of multiple variant domains to rapidly create libraries that enable high-throughput screening and characterization of novel and known variants for therapeutic discovery.
Utilizing your customized, user defined gene parts, Twist Bioscience will create highly uniform combinatorial assembly libraries that you can use to screen for and identify desired combinations of custom gene parts within TCR, CAR or antibody VHH/scFv domains.
TCR Combinatorial Assembly Library With Twist
With arrayed TCR libraries (figure 1a), each user-defined alpha and beta chains are uniquely paired with one another to mimic the natural hit repertoire, where as with combinatorial TCR libraries (figure 1b), the alpha and beta chains are shuffled together to create a library of 16 different combinations, allowing for a library of greater diversity to interrogate the variant space beyond the identified repertoire.
Combinatorial TCR Library Data
Arrayed TCR Library Data
Under discovery of TCRs is driven by the screening of immune cell repertoires and the re-synthesis of large numbers of potential binders. Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening. With arrayed TCR libraries, the alpha and beta chains are shuffled together to create a library of greater diversity to integrate the variant space beyond the identified repertoire.
We will provide you with highly uniform screening libraries that are precise, user-defined combinations of gene fragments, which enable you to efficiently test comprehensive screening of the desired combinations.
Tell us what you would like more information on. We’re here to help!
Contact Us
Combinatorial Assembly Libraries
Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR), chimeric antigen receptor (CAR), and multispecific VHH libraries all through our combinatorial assembly libraries.
Combinatorial Assembly Libraries utilize seamless assembly of multiple variant domains to rapidly create libraries that enable high-throughput screening and characterization of novel and known variants for therapeutic discovery.
Utilizing your customized, user defined gene parts, Twist Bioscience will create highly uniform combinatorial assembly libraries that you can use to screen for and identify desired combinations of custom gene parts within TCR, CAR or antibody VHH/scFv domains.
TCR Combinatorial Assembly Library With Twist
With arrayed TCR libraries (figure 1a), each user-defined alpha and beta chains are uniquely paired with one another to mimic the natural hit repertoire, where as with combinatorial TCR libraries (figure 1b), the alpha and beta chains are shuffled together to create a library of 16 different combinations, allowing for a library of greater diversity to interrogate the variant space beyond the identified repertoire.
Combinatorial TCR Library Data
Arrayed TCR Library Data
Under discovery of TCRs is driven by the screening of immune cell repertoires and the re-synthesis of large numbers of potential binders. Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening. With arrayed TCR libraries, the alpha and beta chains are shuffled together to create a library of greater diversity to integrate the variant space beyond the identified repertoire.
We will provide you with highly uniform screening libraries that are precise, user-defined combinations of gene fragments, which enable you to efficiently test comprehensive screening of the desired combinations.
Tell us what you would like more information on. We’re here to help!
Contact Us
Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening and the identification of TCRs against tumor neoantigens.
Each domain of the CAR scaffold influences the functionality of the CAR both independently and synergistically. Combinatorial Assembly Libraries serve as a tool to fine-tune each of the modules to uncover codependencies and better understand the impact on T-cell specificity, antigen recognition and T-cell function.
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Combinatorial libraries ready for screening that are a realistic dataset comprised of every variable domain possible and are precisely synthesized as a fragment
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Option to swap out scaffold components and test with ease
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Ability to freely shuffle subunits to identify new arrangements that elicit precise and desirable immune responses
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Process smaller component libraries (CARs) and screen against novel targets (TCRs)
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NGS verified libraries so you know exactly which variants are being screened and can proceed to the next iteration of library design even faster
Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening and the identification of TCRs against tumor neoantigens.
Each domain of the CAR scaffold influences the functionality of the CAR both independently and synergistically. Combinatorial Assembly Libraries serve as a tool to fine-tune each of the modules to uncover codependencies and better understand the impact on T-cell specificity, antigen recognition and T-cell function.
-
Combinatorial libraries ready for screening that are a realistic dataset comprised of every variable domain possible and are precisely synthesized as a fragment
-
Option to swap out scaffold components and test with ease
-
Ability to freely shuffle subunits to identify new arrangements that elicit precise and desirable immune responses
-
Process smaller component libraries (CARs) and screen against novel targets (TCRs)
-
NGS verified libraries so you know exactly which variants are being screened and can proceed to the next iteration of library design even faster
Visit our eCommerce platform to create or login to your Twist Bioscience account. Once you have logged into your account on the the eCommerce site, go to "libraires" and click on "Download Combinatorial Assembly Libraries Submission Form." Following, fill out the downloaded submission form and send it to [email protected] for design review. Once the design review is complete, we will provide you with a quote. The price and turnaround time of your combinatorial assembly libraries will vary per project.
Visit our eCommerce platform to create or login to your Twist Bioscience account. Once you have logged into your account on the the eCommerce site, go to "libraires" and click on "Download Combinatorial Assembly Libraries Submission Form." Following, fill out the downloaded submission form and send it to [email protected] for design review. Once the design review is complete, we will provide you with a quote. The price and turnaround time of your combinatorial assembly libraries will vary per project.
