The variant fragments are blunt-ended, double-strand DNA molecules.

Twist has tested the material to be a suitable standard in non-fragmenting end-repair and A-tail ligation (ERAT) library preparation protocols, as well as in amplicon protocols, such as ddPCR and qPCR. The performance of the v2 Pan-cancer Reference Standard is similar to the v1, and to any other dsDNA input into library prep.