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Product Configuration

• What are the kit configurations of the cfDNA Library Preparation Kit?
• What are the shipping and storage conditions of the cfDNA Library Preparation Kit?
• What is the shelf life of the cfDNA Library Preparation Kit?
• Should I worry about freeze/thaw cycles with the cfDNA Library Prep Kit?
• What is not included with the cfDNA Library Preparation Kit?

Sample Input

• How much DNA input can I use with the cfDNA Library Preparation Kit?
• How do I determine how much cfDNA to use?
• Do you recommend any QC assays to perform on the input cfDNA prior to library preparation?
• Can I increase the volume of cfDNA input if my DNA concentration is very low?
• Can I use sample DNA other than cfDNA with this kit?
• Can I only use 1 - 20 ng with the DNA derived from FFPE samples?

Library Preparation

• What are the advantages of the cfDNA Library Preparation kit as compared to other kits?
• How long does it take to construct a library using the cfDNA Library Preparation Kit?
• What kind of sequencing libraries does the cfDNA Library Preparation Kit support?
• Does the cfDNA Library Preparation Kit support Unique Molecular Identifiers (UMIs)?​
• What if I do not need the UMIs with the cfDNA Library Prep kit? Can I use UDI adapters?
• Can I adjust the SPRI ratio after ligation from the recommended 0.9X?
• Why do I see larger peaks in my cfDNA library amplification?

Hybridization

• What plexity does the cfDNA Target Enrichment Kits support?
• How do I determine library mass into target capture?
• How did you derive this 80x multiplier library input into target capture and do I need to follow it?
• How much mass can I add into a singleplex target enrichment
• What will happen if more than 12.8 μg input mass is added into capture?
• How do I set up 8-plex Target Enrichment with libraries made from different sample mass inputs?
• What is the recommendation if I have libraries made from sample mass inputs >20 ng?