How do I determine how much cfDNA to use?
We recommend referencing our datasheet to determine optimal cfDNA mass input:
https://www.twistbioscience.com/resources/technical-document/cfdna-library-prep-kit-datasheet
The optimal amount of cfDNA input should be determined by weighing variables such as desired
sensitivity at target VAF, duplexing requirements, panel content, and available sequencing
resources.
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