What if I do not need the UMIs with the cfDNA Library Prep kit? Can I use UDI adapters?
The kit can prepare cfDNA libraries both with universal adapters for PCR with UDI primers and also with full-length UDI adapters for PCR-free and WGS applications (see cfDNA full-length UDI protocol here:
| Product | Twist UDI Primers – TruSeq Compatible | Twist HT Universal Adapter System | Twist Full Length UDI Adapters |
|---|---|---|---|
| Unique Indexes | 16 or 384 Unique Index | 3,072 Unique Index | 1,536 Full Length UDI Adapter |
| Index Length | 10 bp | 12 bp | 10 bp |
| Concentration | 10 µM | 10 µM | 10 uM |
| Available Format(s) | Tubes or 96-well plates | 96- or 384-well plates | 96-well plates |
| Best For | Most Applications | High-Throughput Applications | PCR-Free WGS |
Refer to https://www.twistbioscience.com/products/ngs/library-preparation/universal-adapter-system for more information.
Additionally, the kit’s molecular biology should work with adapters and primers from other vendors. However, the adapter must contain a 3’ T overhang to facilitate the A/T ligation with the 3’ A overhang in the library molecules.
Final library yield may be slightly lower when not using the Twist-optimized solution.
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