How did you derive this 80x multiplier library input into target capture and do I need to follow it?
We found that library input mass into target enrichment can affect the complexity of deeply sequenced libraries and this multiplier was determined based on empirical data. This approach ensures maximum effectiveness of the enrichment process.
For more details, refer to our cfDNA Library Preparation Kit Datasheet here: https://www.twistbioscience.com/resources/technical-document/cfdna-library-prep-kit-datasheet
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