Enhanced Sensitivity For Rare Variant Detection
Enhanced Sensitivity For Rare Variant Detection
Enhanced Sensitivity For Rare Variant Detection
INFORMACIÓN GENERAL DATOS PEDIDOS Y ESPECIFICACIONES
Descripción general

The Twist UMI Adapter system offers a sensitive method for identifying low frequency somatic variants from cell free DNA (cfDNA). Libraries prepared with the UMI adapter system can be used for building consensus reads during analysis to increase variant detection sensitivity and to reduce PCR error contribution. 

Engineered for optimal performance with heterogenous sample types, the system is available in either 16 or 96 sample formats. It contains:

  • Twist Unique Molecular Identifier (UMI) Adapters - matched unique molecular indices (two for each molecule) that are compatible with 'T-A' overhang workflows.
  • Twist 10 bp Unique Dual-Indexed (UDI) Primers - Designed with unique dual indices for each mixed primer pair and provided in a 96-well plate format suitable for automation applications.
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High Sensitivity
Matched adapters enable superior identification of PCR errors
Reduces false positives of low frequency variants for cancer research and discovery
Compatible
Compatible with Twist’s NGS Solutions
Easy integration with Twist Mechanical Fragmentation library prep workflow
Tested for performance with Twist’s target enrichment panels
Escalable
Appropriate for somatic applications
Accepts gDNA or cfDNA input
Scalable formats for 16 or 96 samples

The Twist UMI Adapter system offers a sensitive method for identifying low frequency somatic variants from cell free DNA (cfDNA). Libraries prepared with the UMI adapter system can be used for building consensus reads during analysis to increase variant detection sensitivity and to reduce PCR error contribution. 

Engineered for optimal performance with heterogenous sample types, the system is available in either 16 or 96 sample formats. It contains:

  • Twist Unique Molecular Identifier (UMI) Adapters - matched unique molecular indices (two for each molecule) that are compatible with 'T-A' overhang workflows.
  • Twist 10 bp Unique Dual-Indexed (UDI) Primers - Designed with unique dual indices for each mixed primer pair and provided in a 96-well plate format suitable for automation applications.
screen
High Sensitivity
Matched adapters enable superior identification of PCR errors
Reduces false positives of low frequency variants for cancer research and discovery
Compatible
Compatible with Twist’s NGS Solutions
Easy integration with Twist Mechanical Fragmentation library prep workflow
Tested for performance with Twist’s target enrichment panels
Escalable
Appropriate for somatic applications
Accepts gDNA or cfDNA input
Scalable formats for 16 or 96 samples
DATOS
Compatible with Twist NGS workflows

Twist’s comprehensive NGS toolkit has what you need to take your low input sample from DNA to data. The Twist UMI adapter system integrates seamlessly into existing library preparation workflows, either as a drop-in replacement for Twist Universal adapters or as an additional step.

Greater sensitivity in variant detection

Twist’s UMI adapter system offers a tool for consensus building that removes errors from PCR duplicates. 

Compatible with Twist NGS workflows

Twist’s comprehensive NGS toolkit has what you need to take your low input sample from DNA to data. The Twist UMI adapter system integrates seamlessly into existing library preparation workflows, either as a drop-in replacement for Twist Universal adapters or as an additional step.

Greater sensitivity in variant detection

Twist’s UMI adapter system offers a tool for consensus building that removes errors from PCR duplicates. 

PEDIDOS Y ESPECIFICACIONES
Códigos de referencia de los productos

105040

Twist UMI Adapter System TruSeq Compatible, 16 Samples

105041

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate A

105042

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate B

105043

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate C

105044

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate D

105094

Twist UMI Adapters - TruSeq Compatible, 96 Samples
Códigos de referencia de los productos

105040

Twist UMI Adapter System TruSeq Compatible, 16 Samples

105041

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate A

105042

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate B

105043

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate C

105044

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate D

105094

Twist UMI Adapters - TruSeq Compatible, 96 Samples