Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers.
Twist Universal Adapter
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist 10 bp Unique Dual-Indexed (UDI) Primer
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist 12bp High Throughput (HT) UDI Primers
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3.072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers.
Twist Universal Adapter
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist 10 bp Unique Dual-Indexed (UDI) Primer
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist 12bp High Throughput (HT) UDI Primers
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3.072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
Die Grafik rechts zeigt, wie die Twist Universal Adapter zur Insertionsligation und UDI-Primer zur PCR-Amplifikation eine robuste Library erstellen.
Die Libraryvorbereitung und das Target Enrichment wirken sich gemeinsam auf die Fähigkeit aus, bei der Sequenzierung Varianten zu erkennen. Es ist für die erfolgreiche Hybrid Capture wichtig, dass konsequent robuste Libraries generiert werden. Wenn Adapter während der Libraryvorbereitung dimerisieren, entstehen unerwünschte Produkte und reduzieren die effektive Konzentration der Adapter, die zur Ligation an Insertionen zur Verfügung stehen. Die Verwendung der Twist Universal Adapter und Primer während der Library-Konstruktion minimiert dieses Problem.
Die Twist Universal Adapter minimieren die Bildung von Adapter-Dimeren während der Libraryvorbereitung.
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
Die Grafik rechts zeigt, wie die Twist Universal Adapter zur Insertionsligation und UDI-Primer zur PCR-Amplifikation eine robuste Library erstellen.
Die Libraryvorbereitung und das Target Enrichment wirken sich gemeinsam auf die Fähigkeit aus, bei der Sequenzierung Varianten zu erkennen. Es ist für die erfolgreiche Hybrid Capture wichtig, dass konsequent robuste Libraries generiert werden. Wenn Adapter während der Libraryvorbereitung dimerisieren, entstehen unerwünschte Produkte und reduzieren die effektive Konzentration der Adapter, die zur Ligation an Insertionen zur Verfügung stehen. Die Verwendung der Twist Universal Adapter und Primer während der Library-Konstruktion minimiert dieses Problem.
Die Twist Universal Adapter minimieren die Bildung von Adapter-Dimeren während der Libraryvorbereitung.
101307
Twist Universal Adapter System TruSeq-kompatibel, 16 Proben101308
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte A101309
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte B101310
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte C101311
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte D106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
101307
Twist Universal Adapter System TruSeq-kompatibel, 16 Proben101308
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte A101309
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte B101310
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte C101311
Twist Universal Adapter System TruSeq-kompatibel, 96 Proben Platte D106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
Sicherheitsdatenblatt
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
Sicherheitsdatenblatt für 101308
Sicherheitsdatenblatt
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
Sicherheitsdatenblatt
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
Sicherheitsdatenblatt für 101307
Sicherheitsdatenblatt
Twist Universal Adapters - TruSeq Comp, 384 Samples (SDS-001125)
Sicherheitsdatenblatt für 104439
Sicherheitsdatenblatt
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
Sicherheitsdatenblatt
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438
Poster
UDI Primers and Universal Blockers Enable Multiplexed Target Enrichments (ASHG 2019)
Sicherheitsdatenblatt
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
Sicherheitsdatenblatt für 101308
Sicherheitsdatenblatt
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
Sicherheitsdatenblatt
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
Sicherheitsdatenblatt für 101307
Sicherheitsdatenblatt
Twist Universal Adapters - TruSeq Comp, 384 Samples (SDS-001125)
Sicherheitsdatenblatt für 104439
Sicherheitsdatenblatt
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
Sicherheitsdatenblatt
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438
Poster
UDI Primers and Universal Blockers Enable Multiplexed Target Enrichments (ASHG 2019)