The Power of Silicon-based DNA Synthesis
Custom antibody products in CHO can be used to help relieve bottlenecks in times of capacity constraints. Let Twist Express Antibodies, CHO, be an extension of your lab to provide you with fast, high-quality, purified antibodies ready for screening. Twist Express Antibodies produced in ExpiCHO™ cells, are harvested and either filtered (Supernatant) or purified using Protein A. The antibodies are then transferred to a preferred shipping container, frozen, and delivered along with the Glycerol Stocks of the individual chains in perfect shape for any functional assay or screening application.
• High-quality antibodies produced from NGS sequence-verified Twist Express Clonal Genes
• Ready to use and scale-up ready using included Glycerol Stock
Twist Express Antibodies can be easily ordered using our integrated eCommerce application with just the Variable (VH & VL) sequences. Please read through our Quick Start Guide for ordering tips. The antibodies can also be thawed using our handling guidelines (here) for use in any functional or screening assays.
Workflow
We've enhanced our antibody offering by leveraging Express Genes for reduced TAT, expanding cell-line offerings and add-ons including normalization, endotoxin testing, and aliquoting to meet your protein production and purification needs.
Performance Data
Figure Description: COVID Panel Expression Levels. Expression Panel of 66 IgGs generated from a COVID Antibody Discovery program. Average yields are represented by the bar while standard deviation is represented by the error bars for approximately 4 replicates of 1 mL expression volumes.
Twist Express Antibodies, CHO Product Offering
Our team is ready to answer all your questions.
The Power of Silicon-based DNA Synthesis
Custom antibody products in CHO can be used to help relieve bottlenecks in times of capacity constraints. Let Twist Express Antibodies, CHO, be an extension of your lab to provide you with fast, high-quality, purified antibodies ready for screening. Twist Express Antibodies produced in ExpiCHO™ cells, are harvested and either filtered (Supernatant) or purified using Protein A. The antibodies are then transferred to a preferred shipping container, frozen, and delivered along with the Glycerol Stocks of the individual chains in perfect shape for any functional assay or screening application.
• High-quality antibodies produced from NGS sequence-verified Twist Express Clonal Genes
• Ready to use and scale-up ready using included Glycerol Stock
Twist Express Antibodies can be easily ordered using our integrated eCommerce application with just the Variable (VH & VL) sequences. Please read through our Quick Start Guide for ordering tips. The antibodies can also be thawed using our handling guidelines (here) for use in any functional or screening assays.
Workflow
We've enhanced our antibody offering by leveraging Express Genes for reduced TAT, expanding cell-line offerings and add-ons including normalization, endotoxin testing, and aliquoting to meet your protein production and purification needs.
Performance Data
Figure Description: COVID Panel Expression Levels. Expression Panel of 66 IgGs generated from a COVID Antibody Discovery program. Average yields are represented by the bar while standard deviation is represented by the error bars for approximately 4 replicates of 1 mL expression volumes.
Twist Express Antibodies, CHO Product Offering
Our team is ready to answer all your questions.
The Power of Silicon-based DNA Synthesis
Custom antibody products in HEK293 can be used to help relieve bottlenecks in times of capacity constraints. Let Twist Express Antibodies, HECK293, be an extension of your lab to provide you with fast, high-quality, purified antibodies ready for screening. Twist Express Antibodies produced in Expi293™ cells, are harvested and either filtered (Supernatant) or purified using Protein A. The antibodies are then transferred to a preferred shipping container, frozen, and delivered along with the Glycerol Stocks of the individual chains in perfect shape for any functional assay or screening application.
• High-quality antibodies produced from NGS sequence-verified Twist Express Clonal Genes
• Ready to use and scale-up ready using included Glycerol Stock
Twist Express Antibodies can be easily ordered using our integrated eCommerce application with just the Variable (VH & VL) sequences. Please read through our Quick Start Guide for ordering tips. The antibodies can also be thawed using our handling guidelines (here) for use in any functional or screening assays.
Workflow
We've enhanced our antibody offering by leveraging Express Genes for reduced TAT, expanding cell-line offerings and add-ons including normalization, endotoxin testing, and aliquoting to meet your protein production and purification needs.
Performance Data
Figure Description: Following antibody production, antibodies were purified and yields were determined. Of the 518 antibodies produced, 482 passed size and pufity QC tests-409 yielded more than 100 ug per antibody.
Twist Express Antibodies, 293 Product Offering
The Power of Silicon-based DNA Synthesis
Custom antibody products in HEK293 can be used to help relieve bottlenecks in times of capacity constraints. Let Twist Express Antibodies, HECK293, be an extension of your lab to provide you with fast, high-quality, purified antibodies ready for screening. Twist Express Antibodies produced in Expi293™ cells, are harvested and either filtered (Supernatant) or purified using Protein A. The antibodies are then transferred to a preferred shipping container, frozen, and delivered along with the Glycerol Stocks of the individual chains in perfect shape for any functional assay or screening application.
• High-quality antibodies produced from NGS sequence-verified Twist Express Clonal Genes
• Ready to use and scale-up ready using included Glycerol Stock
Twist Express Antibodies can be easily ordered using our integrated eCommerce application with just the Variable (VH & VL) sequences. Please read through our Quick Start Guide for ordering tips. The antibodies can also be thawed using our handling guidelines (here) for use in any functional or screening assays.
Workflow
We've enhanced our antibody offering by leveraging Express Genes for reduced TAT, expanding cell-line offerings and add-ons including normalization, endotoxin testing, and aliquoting to meet your protein production and purification needs.
Performance Data
Figure Description: Following antibody production, antibodies were purified and yields were determined. Of the 518 antibodies produced, 482 passed size and pufity QC tests-409 yielded more than 100 ug per antibody.
Twist Express Antibodies, 293 Product Offering
Product Sheet
This document will provide you detailed information on Twist Bioscience’s Express Antibodies - CHO offering, including product specifications, customizations, and more.
Product Sheet
This document will provide you detailed information on Twist Bioscience’s Express Antibodies - HEK293 offering, including product specifications, customizations, and more
Guide/Guideline
Recommended handling and storage instructions for Twist Hight Throughput IgG
Flyer
Twist's platform is designed to provide robust expression of antibody candidates with yields generated for potential antibody hits against a specific antigen. Learn about the expression data Twist will provide you in this flyer.
Application Note
Deep Repertoire Mining Uncovers Ultra-broad Coronavirus-Neutralizing Antibodies using Twist’s High Throughput Antibody Production and SPR Screening
Guide/Guideline
This Quick start guide will walk you through the process of preparing your sequences and placing an order within Twist’s ordering platform.
Product Sheet
This document will provide you detailed information on Twist Bioscience’s Express Antibodies - CHO offering, including product specifications, customizations, and more.
Product Sheet
This document will provide you detailed information on Twist Bioscience’s Express Antibodies - HEK293 offering, including product specifications, customizations, and more
Guide/Guideline
Recommended handling and storage instructions for Twist Hight Throughput IgG
Flyer
Twist's platform is designed to provide robust expression of antibody candidates with yields generated for potential antibody hits against a specific antigen. Learn about the expression data Twist will provide you in this flyer.
Application Note
Deep Repertoire Mining Uncovers Ultra-broad Coronavirus-Neutralizing Antibodies using Twist’s High Throughput Antibody Production and SPR Screening
Guide/Guideline
This Quick start guide will walk you through the process of preparing your sequences and placing an order within Twist’s ordering platform.
To place an order through our eCommerce portal, simply prepare your trimmed variable region sequences according to our Quick start guide and paste them into our upload template along with the corresponding antibody names. Once the file is uploaded, the portal will ask for the IgG subtype, light chain type, expression vectors (Twist provided or custom), and to specify the Kozak and leader sequences to use. Once the sequences are screened and reviewed, simply select the scale (1 mL vs. 8 mL), grade (pure vs. supernatant), elution buffer, buffer exchange (optional), and delivery format to complete your order!
To place an order through our eCommerce portal, simply prepare your trimmed variable region sequences according to our Quick start guide and paste them into our upload template along with the corresponding antibody names. Once the file is uploaded, the portal will ask for the IgG subtype, light chain type, expression vectors (Twist provided or custom), and to specify the Kozak and leader sequences to use. Once the sequences are screened and reviewed, simply select the scale (1 mL vs. 8 mL), grade (pure vs. supernatant), elution buffer, buffer exchange (optional), and delivery format to complete your order!
General
Delivery Format
Resources and Guidance for Sequence Design
-
abYsis provides an integrated, comprehensive, and annotated sequence assessment for one or multiple antibodies. AbYsis provides a thorough regional breakdown of antibody sequences to quickly identify where your variable region starts and ends. A tabular breakdown of the leader, FWK, and CDR sequences as well as the tail, or constant region sequence is also provided.
-
It can be helpful to apply the annotations provided by abYsis to your sequence using bioinformatic programs to help visualize and isolate your variable region. Below is an example of a sequence imported to the Geneious Prime software platform with abYsis annotations applied.
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One additional option that may provide comprehensive breakdown of leader, variable, and constant regions is ANARCI. The resulting analysis provides the exact variable region sequence highlighted in red, which can then be uploaded to the Antibodies application on our eCommerce website. ANARCI also provides detail on the light chain domain type and the sequence species.
-
Please keep in mind that variable region sequences should never start with a methionine (M). If you are uploading sequences that start with the amino acid M, or nucleotides ATG, and are planning to use pTwist vectors, please contact our support for assistance.
-
Reformatting variable domains from one species to another can be complicated given differences between disulfide bonding and other features. By default, the Twist eCommerce platform will not correct any of these issues. We typically rely on you to account and inspect for these type of reformatting complications. If you need assistance, please contact our support team.
General
Delivery Format
Resources and Guidance for Sequence Design
-
abYsis provides an integrated, comprehensive, and annotated sequence assessment for one or multiple antibodies. AbYsis provides a thorough regional breakdown of antibody sequences to quickly identify where your variable region starts and ends. A tabular breakdown of the leader, FWK, and CDR sequences as well as the tail, or constant region sequence is also provided.
-
It can be helpful to apply the annotations provided by abYsis to your sequence using bioinformatic programs to help visualize and isolate your variable region. Below is an example of a sequence imported to the Geneious Prime software platform with abYsis annotations applied.
-
One additional option that may provide comprehensive breakdown of leader, variable, and constant regions is ANARCI. The resulting analysis provides the exact variable region sequence highlighted in red, which can then be uploaded to the Antibodies application on our eCommerce website. ANARCI also provides detail on the light chain domain type and the sequence species.
-
Please keep in mind that variable region sequences should never start with a methionine (M). If you are uploading sequences that start with the amino acid M, or nucleotides ATG, and are planning to use pTwist vectors, please contact our support for assistance.
-
Reformatting variable domains from one species to another can be complicated given differences between disulfide bonding and other features. By default, the Twist eCommerce platform will not correct any of these issues. We typically rely on you to account and inspect for these type of reformatting complications. If you need assistance, please contact our support team.
