The Twist Hyperimmune Library synthetically mimics the human in vivo antibody repertoire, providing optimal diversity for antibody development against any target.
This naïve library is the original design of the Twist Hyperimmune Library series, which also includes the VHH hShuffle Hyperimmune (HI) and Common Light Chain Hyperimmune libraries.
A highly diverse kappa VK1-39 light chain library was paired with the VH3-23 heavy chain library to yield a highly diverse fully human Fab phage display library.
HCDR3 sequence diversities were combinatorially assembled and incorporated into the humanized VH3-23 framework to construct the heavy chain library, which was then paired with a highly diverse kappa VK1-39 light chain library. This combination yields a highly functional antibody library of ~1010 size. Originally offered as a Fab library, the library is now also available in the more compact scFv format.
Offers high diversity human CDR3 with natural llama CDR1/2 sequences in a humanized DP-47 VHH framework. More than two million HCDR3 sequences were gathered from a database of human naïve and memory B-cell receptor sequences and incorporated into the VHH hShuffle library
For the common light chain hyperimmune library, trastuzumab light chain is cloned in place of the VK1-39 light chain library.
LIBRARIES FOR THERAPEUTIC DEVELOPMENT
Learn more about developing diverse synthetic fully human antibody libraries for antiviral therapeutic development.
Proof of Concept Data
Twist’s Hyperimmune Library was panned against the SARS-CoV-2 S1 Spike antigen. A large number of unique clones including TB182-3, -4, and -7, were identified possessing a range of binding affinities. Their activities were demonstrated in competition and functional studies.
Library Panning and Screening: Panning to Functional Assays Using Full-Length IgG
This diverse Hyperimmune Library is screened against target antigens. Functional antibodies can be obtained in 8+ weeks.
Anti-S1 mAbs Kinetics, SARS-CoV-2 (Top Leads): Direct Coupled Antibodies
Twist’s Hyperimmune Library has been effective at uncovering SARS-CoV-2 S1 Protein virus leads as well as broad CD3e
discovery effort.
S1 RBD: VERO E6 Inhibition by FACS
TB182-3 and TB-182-4 Show Potent Inhibition of S1 Binding to ACE2-expressing VERO E6 cells.
Specifications
A synthetic phage antibody library was derived from public databases of naïve and memory B-cell receptor sequences from three human donors. More than two million HCDR3 sequences were gathered and constructed with Twist’s DNA synthesis capabilities.
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Genes
Oligo Pools
NGS
Variant Libraries
Antibody Discovery
IVDR Solutions
