Technical Note
Protocol
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Formalin-Fixed, Paraffin-Embedded (FFPE) Sample Processing for Successful Target Enrichment
A workflow for FFPE sample sequencing combining Covaris' sample extraction solutions with Twist Bioscience’s Target Enrichment Solutions
Library construction for Next Generation Sequencing (NGS) using formalin-fixed paraffin-embedded (FFPE) samples offers unique challenges in acquiring high-quality sequencing data due to wide distribution of sample quality. Differences in formalin fixation methods, storage conditions, and age lead to cross-linked and/or degraded nucleic acids and inconsistent extraction yields. Therefore, FFPE extraction and library construction methods must be carefully considered for target enrichment applications. In collaboration, Covaris and Twist Bioscience demonstrate a complete library generates read-to-sequence multiplexed libraries directly from FFPE tissue of various qualities.
Covered in this Application Note
A complete workflow for target enrichment from FFPE samples
Comparison of sequencing performance following extraction and enrichment from multiple cell types
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