Technical Note
Protocol
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Library Preparation Protocol - Enzymatic Fragmentation and Combinatorial Dual Indices
A comprehensive protocol for the preparation of genomic DNA libraries using enzymatic fragmentation and Y-shaped Adapters
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This protocol is for the preparation of genomic DNA (gDNA) libraries using enzymatic gDNA fragmentation and Y-shaped adapters. This manual details the steps for generating the amplified, indexed libraries needed for downstream target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. This library preparation protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.
Covered in this Protocol
User Supplied Materials and Equipment
General Notes and Precautions
Guidelines for gDNA Samples
Twist Human Core Exome EF Workflow with Combinatorial dual indices
