Technical Note
Protocol
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Twist Sample Preparation Protocol: Covaris FFPE DNA Extraction
Prepare FFPE samples for NGS sequencing with improved yield and quality.
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An optimal NGS workflow starts with an optimal Sample Preparation step, followed by the Library Prep. Sample preparation is a critical step, and often challenging because of the quality of the samples. This is the case with FFPE tissue samples, where the starting material could be degraded. Adaptive Focused Acoustics® (AFA) technology, developed by Covaris for improving the Sample prep step, drastically improves the yield and the quality of extracted nucleic acids. Covaris is the gold standard technology for NGS fragmentation thanks to its unparalleled results.
Covered in this Protocol
General Notes and Precautions
Extraction of gDNA from FFPE Samples
Fragmentation gDNA Using AFA Technology
