Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
CRISPR Screening in Single Cells
PRODUCTS USED
ABSTRACT
The combination of single-cell RNA-seq and CRISPR allows for efficient interrogation of possibly any number of genes, only limited by the sequencing capability. Here we describe the current protocols for CRISPR screening in single cells, from cloning and virus production to generating sequencing data.