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DNA Shuffling using NEBridge® Golden Gate Assembly for Protein Engineering
PRODUCTS USED
ABSTRACT
DNA shuffling is a widely adopted method for recombining sequences to generate chimeric proteins with new or improved properties, such as thermotolerance, tolerance to harsh chemicals, or higher catalytic efficiency. Homologous recombination is typically used with highly similar sequences to scramble genes to make new proteins. These methods have been used to engineer beta-lactamases, P450s, and many more enzymes (1,2).
