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Enzymatic fragmentation enables scalable preparation of high-quality FFPE libraries with minimal artifacts
PRODUCTS USED
ABSTRACT
Improved chemistry and flexible parameters enable consistent fragmentation and control over FFPE library insert size. • Single-tube protocol limits sample loss, improves library complexity and sequencing metrics, and enables full automation. • Minimal artifacts (compared to other enzymatic fragmentation methods and sonication) facilitate data interpretation and improves sequencing economy.