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Evolving Escherichia coli to use a tRNA with a non-canonical fold as an adaptor of the genetic code
PRODUCTS USED
ABSTRACT
All known bacterial tRNAs adopt the canonical cloverleaf 2D and L-shaped 3D structures. We aimed to explore whether alternative tRNA structures could be introduced in bacterial translation. To this end, we crafted a vitamin-based genetic system to evolve Escherichia coli toward activity of structurally non-canonical tRNAs. The system reliably couples (escape frequency
