Genome wide screen of RNAi molecules against SARS-CoV-2 creates a broadly potent prophylaxis

bioRxiv : the preprint server for biology, Apr 2022

DOI: 10.1101/2022.04.12.488010

Genome wide screen of RNAi molecules against SARS-CoV-2 creates a broadly potent prophylaxis

Yogev, Ohad ; Weissbrod, Omer ; Battistoni, Giorgia ; Bressan, Dario ; Naamti, Adi ; Falciatori, Ilaria ; Berkyurek, Ahmet C ; Rasnic, Roni ; Hosmillo, Myra ; Ilan, Shaul ; Grossman, Iris ; McCormick, Lauren ; Honeycutt, Christopher C ; Johnston, Timothy ; Gagne, Matthew ; Douek, Daniel C ; Goodfellow, Ian ; Hannon, Gregory J ; Erlich, Yaniv

PRODUCTS USED

Oligo Pools

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ABSTRACT

Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is of utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen with over 16,000 RNAi triggers against the SARS-CoV-2 genome using a massively parallel assay to identify hyper-potent siRNAs. We selected 10 candidates for in vitro validation and found five siRNAs that exhibited hyper-potent activity with IC50

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PRODUCTS USED

Oligo Pools

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery