From a genome-wide screen of RNAi molecules against SARS-CoV-2 to a validated broad-spectrum and potent prophylaxis

Communications biology, Mar 2023 | 6 (1), 277

DOI: 10.1038/s42003-023-04589-5

From a genome-wide screen of RNAi molecules against SARS-CoV-2 to a validated broad-spectrum and potent prophylaxis

Yogev, Ohad ; Weissbrod, Omer ; Battistoni, Giorgia ; Bressan, Dario ; Naamati, Adi ; Falciatori, Ilaria ; Berkyurek, Ahmet Can ; Rasnic, Roni ; Izuagbe, Rhys ; Hosmillo, Myra ; Ilan, Shaul ; Grossman, Iris ; McCormick, Lauren ; Honeycutt, Christopher Cole ; Johnston, Timothy ; Gagne, Matthew ; Douek, Daniel C ; Goodfellow, Ian ; Hannon, Gregory James ; Erlich, Yaniv

PRODUCTS USED

Oligo Pools

Read Full Article

ABSTRACT

Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is of utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen of over 16,000 RNAi triggers against the SARS-CoV-2 genome, using a massively parallel assay to identify hyper-potent siRNAs. We selected Ten candidates for in vitro validation and found five siRNAs that exhibited hyper-potent activity (IC50

Read Full Article

PRODUCTS USED

Oligo Pools

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery