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    Clonal Genes

    • Can I get a glycerol stock of my clonal gene?
    • Can Twist provide cloned products?
    • Do Twist Cloning or Expression vectors come with a Kozak and signal peptide sequence included in the vector backbone?
    • Does Twist offer plant expression vectors?
    • What is the price for a clonal gene?
    • Where can I find a Certificate of Analysis (COA) for a clonal gene?

    Codon Optimization

    • For codon optimization, what steps are taken to maintain wild-type protein expression levels?
    • If my gene is scored as Impossible, can I still have it synthesized?
    • Which organisms does Twist currently support for codon optimization?

    Express Service

    • Can I order all of my sequences as Express Genes?
    • Do advertised Express Gene turnaround times include shipping time?
    • Does the Express Gene service turnaround time include new custom vector onboarding?
    • How does Twist Bioscience manufacture Express Genes so fast?
    • How will the Express fee be applied if I have contracted pricing?
    • I received the below message when trying to place my Express Genes order, what does this flag mean?
    • Is there an additional fee for the Express service?
    • Is there an order maximum for Express Genes?
    • What are Express Genes? Is it the same as Clonal Genes with the Express service?
    • What are the differences between the Express and Standard Clonal Gene offerings?
    • What are the sequence acceptance criteria for Express Genes?
    • What DNA scale and format options are available for Express Genes?
    • What does it mean if the Express icon is grayed out for a sequence?
    • What if I do not want to pay the additional fee for Express Genes?
    • What if my Express Genes do not ship on-time or I am not satisfied with my Express Genes order?
    • Why does the pricing for Express Genes change?
    • Why don’t complex sequences qualify for the Express service?

    Gene Fragments

    • After cloning, how many colonies should I pick to increase my chances of finding a clone with my correct sequence?
    • Are adapter sequences appended onto the ends of my sequences?
    • Are Gene Fragments purified?
    • What are the adapter sequences used on the Adapter-On Gene Fragments?
    • What is the price for a gene fragment?
    • What methods can I use to clone my adapter-on gene fragments?
    • Where can I find a Certificate of Analysis (COA) for a gene fragment?

    General

    • Do I need to include restriction sites or overhangs on my sequences?
    • Does Twist meet biosecurity screening requirements for the United States?
    • How are the genes delivered?
    • How do I find out the arrangement of my genes in your 96-well plate?
    • How does Twist test the quality of the gene synthesis?
    • How is processing different when my gene is delivered in a plate versus having it delivered in a tube?
    • How much of my DNA is delivered?
    • How should I resuspend my genes?
    • I got my genes, but the yield says “NA”. What does that mean?
    • What is Animal Origin Free?
    • What is the difference between Twist's Clonal Genes and Gene Fragments?
    • What is the maximum size gene I can purchase?
    • What is the minimum size gene I can purchase?
    • What is the shelf life of Gene Fragments and Clonal Genes?
    • What is the Target Yield option?
    • What is the Turn Around Time (TAT) for gene synthesis?
    • Where can I find an SDS for gene products?

    Sequence Design

    • Are there any sequence limitations/design guidelines for genes which I should follow?
    • How do I convert my Amino Acid sequence to a nucleic acid sequence?
    • What do the scoring results of my gene mean?
    • What lengths of synthetic DNA does Twist currently offer for synthesis?
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