Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Targeted mitochondrial genome coverage for sensitive variant detection
Achieve high-depth sequencing of mitochondrial DNA (mtDNA) to detect variants and heteroplasmy with confidence. The Twist Mitochondrial Panel is a fixed content panel designed to cover all 16,659 base pairs (bp) and 37 genes of the mitochondrial genome. This targeted panel enables accurate analysis of mitochondrial biology, population studies, and disease research with efficient enrichment and consistent coverage across the mitochondrial genome.
High uniformity for mtDNA sequencing
Low fold-80 values ensure uniform coverage, improving mitochondrial variant detection and sequencing efficiency.
Flexible standalone or spike-in use
Use independently or combine with exome panels to expand genomic analysis without compromising performance.
Optimized for consistent performance
Engineered probes deliver balanced GC coverage and reproducible enrichment across all mitochondrial targets.
Product data
A critical component for successful targeted sequencing analysis is the quality of the capture reaction, which drives the uniformity of reads. A more uniform capture leads to more uniform sequencing, as indicated by lower fold-80 base penalty scores. With higher uniformity, sequencing reads can effectively drive on-target coverage, limiting wasted reads. High uniformity is the combined result of the probe design algorithm, silicon-based synthesis of dsDNA probes, and calibration processes that ensure uniform amplification, GC content, and enrichment performance across all targets. NGS-based quality control of all Twist Panels ensures that all probes are present at the correct concentrations and that the reproducibility of results is independent of our production processes.
Graph shows the Twist Mitochondrial Panel spiked into Twist Core Exome. Data obtained with 2.3 Mb (150X) sequencing. Complete coverage with excellent uniformity and sequencing efficiency is demonstrated.
The Twist Mitochondrial Panel can serve as added content to the Twist Human Core & Comprehensive Exome Panels, a Twist Custom Panel, or used as a standalone panel.
Video
Uniform coverage improves sequencing efficiency
A critical component for successful targeted sequencing analysis is the quality of the capture reaction, which drives the uniformity of reads. A more uniform capture leads to more uniform sequencing, as indicated by lower fold-80 base penalty scores. With higher uniformity, sequencing reads can effectively drive on-target coverage, limiting wasted reads. High uniformity is the combined result of the probe design algorithm, silicon-based synthesis of dsDNA probes, and calibration processes that ensure uniform amplification, GC content, and enrichment performance across all targets. NGS-based quality control of all Twist Panels ensures that all probes are present at the correct concentrations and that the reproducibility of results is independent of our production processes.
Graph shows the Twist Mitochondrial Panel spiked into Twist Core Exome. Data obtained with 2.3 Mb (150X) sequencing. Complete coverage with excellent uniformity and sequencing efficiency is demonstrated.
Mitochondrial panel spike-in performance
The Twist Mitochondrial Panel can serve as added content to the Twist Human Core & Comprehensive Exome Panels, a Twist Custom Panel, or used as a standalone panel.
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Uniform coverage improves sequencing efficiency
A critical component for successful targeted sequencing analysis is the quality of the capture reaction, which drives the uniformity of reads. A more uniform capture leads to more uniform sequencing, as indicated by lower fold-80 base penalty scores. With higher uniformity, sequencing reads can effectively drive on-target coverage, limiting wasted reads. High uniformity is the combined result of the probe design algorithm, silicon-based synthesis of dsDNA probes, and calibration processes that ensure uniform amplification, GC content, and enrichment performance across all targets. NGS-based quality control of all Twist Panels ensures that all probes are present at the correct concentrations and that the reproducibility of results is independent of our production processes.
Mitochondrial panel spike-in performance
The Twist Mitochondrial Panel can serve as added content to the Twist Human Core & Comprehensive Exome Panels, a Twist Custom Panel, or used as a standalone panel.
For Research Use Only. Not for use in diagnostic procedures.
