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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Targeted mitochondrial genome coverage for sensitive variant detection
Achieve high-depth sequencing of mitochondrial DNA to detect variants and heteroplasmy with confidence. This targeted panel enables accurate analysis of mitochondrial disorders, population studies, and disease research with efficient enrichment and consistent coverage across the mitochondrial genome.
High uniformity for mtDNA sequencing
Low fold-80 values ensure uniform coverage, improving mitochondrial variant detection and sequencing efficiency.
Flexible standalone or spike-in use
Use independently or combine with exome panels to expand genomic analysis without compromising performance.
Optimized for consistent performance
Engineered probes deliver balanced GC coverage and reproducible enrichment across all mitochondrial targets.
Product data
A critical component for successful targeted sequencing analysis is the quality of the capture reaction, which drives the uniformity of reads. A more uniform capture leads to more uniform sequencing, as indicated by lower fold-80 base penalty scores. With higher uniformity, sequencing reads can effectively drive on-target coverage, limiting wasted reads. High uniformity is the combined result of the probe design algorithm, silicon-based synthesis of dsDNA probes, and calibration processes that ensure uniform amplification, GC content, and enrichment performance across all targets. NGS-based quality control of all Twist Panels ensures that all probes are present at the correct concentrations and that the reproducibility of results is independent of our production processes.
Graph shows the Twist Mitochondrial Panel spiked into Twist Core Exome. Data obtained with 2.3 Mb (150X) sequencing. Complete coverage with excellent uniformity and sequencing efficiency is demonstrated.
The Twist Mitochondrial Panel can serve as added content to the Twist Human Core & Comprehensive Exome Panels, a Twist Custom Panel, or used as a standalone panel.
Sequencing results with the Mitochondrial Panel as a spike-in with the Twist Human Core Exome at different concentrations demonstrate similar coverage indicative of uniformity irrespective of varying concentrations.
Video
Uniform coverage improves sequencing efficiency
A critical component for successful targeted sequencing analysis is the quality of the capture reaction, which drives the uniformity of reads. A more uniform capture leads to more uniform sequencing, as indicated by lower fold-80 base penalty scores. With higher uniformity, sequencing reads can effectively drive on-target coverage, limiting wasted reads. High uniformity is the combined result of the probe design algorithm, silicon-based synthesis of dsDNA probes, and calibration processes that ensure uniform amplification, GC content, and enrichment performance across all targets. NGS-based quality control of all Twist Panels ensures that all probes are present at the correct concentrations and that the reproducibility of results is independent of our production processes.
Graph shows the Twist Mitochondrial Panel spiked into Twist Core Exome. Data obtained with 2.3 Mb (150X) sequencing. Complete coverage with excellent uniformity and sequencing efficiency is demonstrated.
Mitochondrial panel spike-in performance
The Twist Mitochondrial Panel can serve as added content to the Twist Human Core & Comprehensive Exome Panels, a Twist Custom Panel, or used as a standalone panel.
Consistent coverage across concentrations
Sequencing results with the Mitochondrial Panel as a spike-in with the Twist Human Core Exome at different concentrations demonstrate similar coverage indicative of uniformity irrespective of varying concentrations.
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Uniform coverage improves sequencing efficiency
A critical component for successful targeted sequencing analysis is the quality of the capture reaction, which drives the uniformity of reads. A more uniform capture leads to more uniform sequencing, as indicated by lower fold-80 base penalty scores. With higher uniformity, sequencing reads can effectively drive on-target coverage, limiting wasted reads. High uniformity is the combined result of the probe design algorithm, silicon-based synthesis of dsDNA probes, and calibration processes that ensure uniform amplification, GC content, and enrichment performance across all targets. NGS-based quality control of all Twist Panels ensures that all probes are present at the correct concentrations and that the reproducibility of results is independent of our production processes.
Mitochondrial panel spike-in performance
The Twist Mitochondrial Panel can serve as added content to the Twist Human Core & Comprehensive Exome Panels, a Twist Custom Panel, or used as a standalone panel.
Consistent coverage across concentrations
Sequencing results with the Mitochondrial Panel as a spike-in with the Twist Human Core Exome at different concentrations demonstrate similar coverage indicative of uniformity irrespective of varying concentrations.
