| TrueAmp Library Preparation Kit |
| Exome 2.0 |
| EM-seq v2 Methylation Detection System |
| cfDNA Pan-Cancer Reference Standard v2 |
| PCR-Free WGS Library Prep Kit |
| Human Methylome Panel |
| Multiplexed Gene Fragments |
| Cloned Oligo Pools |
| Site Saturation Libraries |
| Spread Out Low Diversity Libraries |
| MRD Express |
| Twist Wallet |
| Manufacturability Analyzer |
| Fast Hybridization Solution, Element Trinity Compatible |
| Tris-HCl buffer, pH 8.0 |
| 0.5 M EDTA, pH 8.0 |
| magnesium chloride (MgCl2) |
| dimethyl sulfoxide (DMSO) |
| sodium dodecyl sulfate |
| 5-methylcytosine |
| deoxynucleotide triphosphates (dNTPs) |
| streptavidin-coated magnetic beads |
| proteinase K |
| guanidine thiocyanate |
| GC-rich and AT-rich regions |
| Tween-20 and 2-mercaptoethanol |
| 80% ethanol wash |
| polyethylene glycol (PEG) |
| <em>Escherichia coli</em> |
| <em>Saccharomyces cerevisiae</em> |
| <em>Sequoia sempervirens</em> |
| <em>Felis catus</em> |
| <em>Arabidopsis thaliana</em> |
| <em>in vitro</em> antibody discovery |
| <em>in vivo</em> and <em>in silico</em> screening |
| <em>de novo</em> gene synthesis |
| <em>BRCA1</em> and <em>TP53</em> variants |
| <em>ACE2</em> and <em>KRAS</em> targets |
| the <em>lacZ</em> reporter gene |
| Dr. Grace Bell, Chief Scientific Officer |
| Mark Frank, Senior Field Application Scientist |
| reviewed by Bill Rich and April Long |
| Lead Strain Engineer, Stealth Mode Biotechnology Company |
| Contact your Twist Field Applications Scientist |
| Illumina and Element Biosciences platforms |
| compatible with Element Trinity |
| Twist Bioscience and NGS ProLab Certified Services |
| Great Place to Work-Certified |
| IVDR-compliant solutions from Twist |
| The Twist TrueAmp Library Preparation Kit enables high uniformity across GC-rich, AT-rich, and repeat-dense regions. Pair it with the Fast Hybridization Solution to shorten your target enrichment workflow to a single day. |
| Order Exome 2.0, the Human Methylome Panel, or a Custom Panel directly from the Twist catalog. All panels are compatible with the TrueAmp Polymerase Mix and the PCR-Free WGS Library Prep Kit. |
| Twist Gene Pools and Oligo Pools let you screen thousands of AI-designed sequences in a single experiment, reducing cost per variant while preserving uniformity. |
| Use the Sequence Design Workbench to run the Manufacturability Analyzer and the Codon Optimizer before submitting your order through TAPI. |
| Resuspend the beads in 50 uL of 10 mM Tris-HCl (pH 8.0) containing 0.1 mM EDTA. Add 2 uL of proteinase K and incubate at 37 C for 30 minutes. |
| The hybridization buffer contains dimethyl sulfoxide (DMSO), betaine, and formamide to improve capture of GC-rich targets. |
| Wash twice with 80% ethanol, then elute in nuclease-free water. Do not allow the streptavidin-coated magnetic beads to dry completely. |
| cfDNA inputs as low as 1 ng are supported when using the cfDNA Library Prep Kit with TrueAmp Polymerase Mix. |
| Variant libraries were expressed in <em>Escherichia coli</em> and validated <em>in vitro</em> before <em>in vivo</em> confirmation in <em>Mus musculus</em>. |
| Researchers at Phytoform Labs screened thousands of AI-designed sequences in <em>Arabidopsis thaliana</em>, accelerating trait engineering for crop improvement. |
| Whole exome sequencing of <em>Felis catus</em> revealed <em>TP53</em> variants absent from the reference genome. |
| Complex Gene Synthesis in 15 business days. Starting at 12 cents per base. |
| Order 5,000 clones at 0.5 ng/uL in a 96-well plate format. |
| <span class="notranslate">Oligo Pools</span> and <span class="notranslate">Exome 2.0</span> are available now. |
| Learn more > Explore now > See it in action |
| Twist offers a 15-day turnaround; competitors average 25 days. |
| When your order contains challenging DNA, Twist helps you keep projects on track. Complex Gene Synthesis in 15 business days. |
| Twist cfDNA Pan-Cancer Reference Standard v2 for liquid biopsy research. |
| Compare Whole Genome Sequencing and Whole Exome Sequencing target enrichment workflows. |
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