SPECIFICATIONS
| Workflow Step | Target Enrichment |
| Format | dsDNA |
| Panel size | 3.6 Mb |
| Content | Protein coding genes |
| Design Databases | CCDS, RefSeq, GENCODE, UCSC |
| Human Reference Genome | hg38 |
| Storage | Store at -5 to -30°C |
- OVERVIEW
- DATA
- ORDERING & SPECS
- RESOURCES
Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.
Twist Library Preparation EF Kit (Enzymatic Fragmentation) incorporates the
reagents and enzymes needed for DNA fragmentation with those for end repair,
dA-tailing, and adapter ligation into a single reaction.
- Ideal for automated, high-throughput library preparation
- Produces tunable, reproducible fragment sizes
- Minimizes sequence bias, maximizes coverage depth
BENEFITS
Accommodate Either Enzymatic or Mechanical Fragmentation
- Use enzymatic fragmentation for automated, high-throughput gDNA
- Use mechanical fragmentation for DNA input of varying types and quality
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Accommodate Either Whole Genome Sequencing or Targeted Enrichment
- Use with Twist full-length CD adapters for PCR-free library Preparation
- Use with the Twist Universal Adapter (UDI) System for maximum yield
Streamline Your Workflow
- Optimized for use in the Twist Targeted NGS workflow
- Flexible DNA sample input from 1 ng – 1 µg (enzymatic)
- Convert DNA samples into robust, amplified libraries in under 2.5 hours
- Combine library preparation steps into a single reaction for improved efficiency and consistent results
Superior coverage uniformity
Figure 1 and 2 show target coverage and Fold-80 with the Twist Human Core Exome Kit and Core Exome + Twist Human RefSeq Panel compared to comparable kits on the market. As seen in both figures, target coverage (>94%) and Fold-80 (1.34–1.36) are far superior using either the Exome only and the Exome + RefSeq Panel compared to other market equivalent kits.
PRODUCT SKUS
101059: Twist Library Preparation EF Kit, Enzymatic Fragmentation, 16 Samples
101058: Twist Library Preparation EF Kit, Enzymatic Fragmentation, 96 Samples
Reagents, enzymes, and purification beads required for enzymatic
fragmentation of gDNA and library construction.
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
