Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries
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HIGHLY COMPATIBLE
5’ and 3’ Adapters as standard
Amplify all Gene Fragments with a single primer pair
Compatible with most cloning methods
Testimonials
We at Clariant onboarded a variety of our internal vectors for various host organisms at Twist Bioscience. We make use of Twist Bioscience's high success rate and fast turnover times of gene synthesis and custom cloning. Our goal is to outsource most of our routine molecular biology work to focus our research force and speed up our development times.
Dr. HELGE JOCHENS
Clariant
The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies.
DR. AMANDA PORTILLO
Institute of Systems and Synthetic Biology
This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.
DR. PHILIPPE MARLIERE
Institute of Systems and Synthetic Biology
The combination of price and service allows us to do science that we could not otherwise do.
The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies.
...This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.
Reduce costs with high quality Gene Fragments
Accurate fragment synthesis accelerates discovery. Twist Bioscience gene fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.
Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.
Fragment Length
300-1,800 bp
Delivery format
Lyophilized dsDNA
Error rate
1:3000
Yield
100 ng - 1 μg per fragment
Order Minimum
1 fragment
Turnaround Time
6-9 business days
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Genes
Oligo Pools
NGS
Variant Libraries
Antibody Discovery
IVDR Solutions
