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South San Francisco, CA 94080
Genes
Complex DNA.
Simple everything else.
Complex features we support
Our Gene Synthesis platform helps you make difficult sequences in predictable timescales. See which complex features we can support below.
Product data
Complex DNA. Simple pricing.
With prices starting at 12 cents per base, you get more complex DNA for your budget. Cost-per-base scales with sequence length, not sequence complexity, so pricing stays predictable. And, with early access, you get an immediate quote for your Complex Genes in our ordering portal.
See pricing 
US manufacturing
All Gene products are USA-made, shipping globally from our factory in Wilsonville, OR. Here, our scientists use ultra-long oligo synthesis on silicon, automated enzymatic assembly, and long-read sequencing to produce your Complex Gene Synthesis projects.
Reliable turnaround
For Complex Genes, we provide an estimated turnaround time before you order as well as live manufacturing tracking on our portal. We aim to complete all Complex Gene orders within 15 business days. We will contact you immediately should challenges arise.
Support from real scientists
Twist’s Technical and Customer Support teams are available to help you tackle your biggest research challenges. If you ever have a question or issue, click on the chat icon in the bottom-right of our website, and connect with a real scientist in minutes.
*For Research Use Only. Not for Diagnostic Procedures.
The prices listed apply exclusively to orders placed within our direct service countries. For regions serviced by our authorized distributors, pricing, taxes, and shipping fees may vary. Customers located in distributor-managed territories are encouraged to contact their local representative directly for an accurate quote and availability.
*For Research Use Only. Not for Diagnostic Procedures.
*We will aim to ship all complex genes within 15 business days.
*For Research Use Only. Not for Diagnostic Procedures.
Product data
Complex DNA. Simple pricing.
Manage projects with predictable costs. While Complex Genes cost a little more than standard gene synthesis, we don't change our cost-per-base on a per-sequence basis. Get an immediate quote for your Complex Genes order in our ordering portal.
See pricing
*For Research Use Only. Not for Diagnostic Procedures.
The prices listed apply exclusively to orders placed within our direct service countries. For regions serviced by our authorized distributors, pricing, taxes, and shipping fees may vary. Customers located in distributor-managed territories are encouraged to contact their local representative directly for an accurate quote and availability.
US manufacturing
All Gene products are USA-made, shipping globally from our factory in Wilsonville, OR. Here, our scientists use ultra-long oligo synthesis on silicon, automated enzymatic assembly, and long-read sequencing to produce your Complex Gene Synthesis projects.
*For Research Use Only. Not for Diagnostic Procedures.
Reliable turnaround
For Complex Genes, we will provide estimated turnaround time before you order as well as live manufacturing tracking on our portal. We will aim to complete all Complex Gene orders within 15 business days. We will contact you immediately should challenges arise.
*We will aim to ship all complex genes within 15 business days.
Support from real scientists
Twist's Technical Support team is available to help you tackle your biggest research challenges. If you ever have a question or issue, click on the chat icon in the bottom-right of our website, and connect with a real scientist in minutes.
*For Research Use Only. Not for Diagnostic Procedures.
How to get simple, transparent ordering
for Complex Genes
FAQ
Can I order all of my sequences as Express Genes?
Clonal Gene sequences scored as Low or Medium complexity are eligible for the Express service. The Twist Bioscience eCommerce platform will score all sequences once they are uploaded and flag which ones are eligible for the Express service.
What are the sequence acceptance criteria for Express Genes?
We use a three-tier scoring system to determine the manufacturability and turnaround time of your genes:
Low Complexity: Standard designs with predictable manufacturing and our fastest turnaround.
Medium Complexity: Designs that require additional manufacturing precautions but do not require changes to your sequence.
High Complexity: Highly intricate designs requiring specialized workflows and extended turnaround times.
Modify: This status appears if your sequence falls outside our complexity parameters, is too long/short, or contains non-standard characters.
Our algorithm flags sequences as High Complexity if they meet any of the following criteria:
GC Content: Local GC content within a 50 bp window falls below 10% or exceeds 90%.
Repeats: Direct repeats >200 bp; Short Tandem Repeats (STRs) >100 bp.
Secondary Structures: Hairpin-forming repeats >100 bp.
Homopolymers: Any single-nucleotide run (A, T, G, or C) >30 bp.
Length: Any sequence between 300 bp and 7000 bp that triggers the above flags.
Note: For Medium and High complexity sequences, our website will now display specific warning flags to show you exactly which features are driving the complexity score.
Low Complexity: Standard designs with predictable manufacturing and our fastest turnaround.
Medium Complexity: Designs that require additional manufacturing precautions but do not require changes to your sequence.
High Complexity: Highly intricate designs requiring specialized workflows and extended turnaround times.
Modify: This status appears if your sequence falls outside our complexity parameters, is too long/short, or contains non-standard characters.
Our algorithm flags sequences as High Complexity if they meet any of the following criteria:
GC Content: Local GC content within a 50 bp window falls below 10% or exceeds 90%.
Repeats: Direct repeats >200 bp; Short Tandem Repeats (STRs) >100 bp.
Secondary Structures: Hairpin-forming repeats >100 bp.
Homopolymers: Any single-nucleotide run (A, T, G, or C) >30 bp.
Length: Any sequence between 300 bp and 7000 bp that triggers the above flags.
Note: For Medium and High complexity sequences, our website will now display specific warning flags to show you exactly which features are driving the complexity score.
What are the differences between the Express and Standard Clonal Gene offerings?
The Express service is an upgraded service for Clonal Genes that provides a faster turnaround time than our Standard service.
Express: 4-7 business days turnaround; DNA Scales: 1 µg - 4 µg, 4 µg - 10 µg, 10 µg - 100 µg, 100 µg - 1 mg (turnaround for 10 µg - 100 µg and 100 µg - 1 mg is 7-10 business days); Complexity: Low and Medium only; Size: 0.3 - 7.0 kb; Vectors: Catalog and Custom; Antibiotics: AMP, KAN, CHL.
Standard: 10 business days turnaround; Same DNA Scales; Complexity: High, Medium or Low ; Size: 0.3 - 7.0 kb; Vectors: Catalog and Custom; Antibiotics: AMP, KAN, BLS, CHL, SPE, TET, ZEO.
Express: 4-7 business days turnaround; DNA Scales: 1 µg - 4 µg, 4 µg - 10 µg, 10 µg - 100 µg, 100 µg - 1 mg (turnaround for 10 µg - 100 µg and 100 µg - 1 mg is 7-10 business days); Complexity: Low and Medium only; Size: 0.3 - 7.0 kb; Vectors: Catalog and Custom; Antibiotics: AMP, KAN, CHL.
Standard: 10 business days turnaround; Same DNA Scales; Complexity: High, Medium or Low ; Size: 0.3 - 7.0 kb; Vectors: Catalog and Custom; Antibiotics: AMP, KAN, BLS, CHL, SPE, TET, ZEO.
What does it mean if the Express icon is grayed out for a sequence?
Only Low and Medium complexity sequences are eligible for the Express service. If a gene sequence is grayed out under the Express Genes section, that means it is a High complexity or needs to be modified (score appears as "Modify") sequence and will be marked as such in the eCommerce platform.
How do I upload my sequences to the Twist platform?
Our updated uploader is designed for maximum flexibility. You can upload an Excel template or a CSV file containing your sequence names and the sequences themselves.
Auto-Detection: The algorithm automatically detects if your sequences are in DNA or Amino Acid format.
Flexible Mapping: The system automatically identifies columns for Well, Plate, and Custom Plate Maps.
Post-Upload Editing: If your file doesn't contain location data, don't worry—you can specify the Well and Plate information directly in the interface after the file has been uploaded.
Auto-Detection: The algorithm automatically detects if your sequences are in DNA or Amino Acid format.
Flexible Mapping: The system automatically identifies columns for Well, Plate, and Custom Plate Maps.
Post-Upload Editing: If your file doesn't contain location data, don't worry—you can specify the Well and Plate information directly in the interface after the file has been uploaded.
Will my codon-optimized sequence be manufacturable?
We have decoupled manufacturability parameters from the core optimization to focus primarily on protein expression. However, if the algorithm cannot find a manufacturable solution, it will automatically attempt to fix localized regions of extreme complexity. We do this while minimizing any impact on the expression profile.
Important: While we optimize for the best possible DNA composition, please remember that protein expression is multifactorial; DNA sequence is just one of many variables influencing your final yield.
Important: While we optimize for the best possible DNA composition, please remember that protein expression is multifactorial; DNA sequence is just one of many variables influencing your final yield.
Request early access to Complex Genes
Fill out the form, and one of our team will be in touch to provide access to Complex Genes ordering in your account.
