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Improve sequencing accuracy and multiplexing with dual indexing adapters
Full-length unique dual indexing (UDI) adapters are designed to enhance next generation sequencing performance by reducing index hopping and sample misassignment. Their optimized structure supports accurate demultiplexing, improved data quality, and confident variant detection. Compatible with a wide range of library preparation workflows, they enable scalable multiplexing and efficient sequencing across research applications.
Minimize index hopping and cross-talk
Full-length unique dual indexes reduce index misassignment and sample cross-contamination, ensuring accurate demultiplexing and reliable variant detection in multiplexed NGS workflows.
Increase data quality and confidence
Optimized adapter design supports high-fidelity sequencing with fewer errors, improving downstream analysis for applications such as rare variant detection and low-frequency mutation analysis.
Scalable for high-throughput sequencing
Supports high levels of multiplexing with a broad index set, enabling efficient sample pooling and cost-effective sequencing across large-scale studies.
Product data
Libraries generated from 300 ng, 75 ng, and 37.5 ng DNA all reach usable concentrations, demonstrating reliable library yield even as input decreases.
Low DNA input has usable library yield. Twist PCR-Free WGS Library Prep Kit libraries with Full Length UDI Adapters for PCR-free WGS were prepared using NA12878 300 ng, 75 ng, and 37.5 ng genomic DNA . Fragmentation parameters were set at 15 minutes and 25°C. Libraries were quantified by qPCR using the Kapa Library Quantification kit.
Estimated library size remains high from 300 ng down to 37.5 ng DNA input, indicating maintained library diversity and strong performance even with lower starting material.
Library diversity is maintained even with low DNA input. Twist PCR-Free WGS Library Preparation Kit libraries with Full Length UDI Adapters for PCR-free WGS were prepared using NA12878 300 ng, 75 ng, and 37.5 ng genomic DNA . Fragmentation parameters were set at 15 minutes and 25°C. Libraries were quantified by qPCR using the Kapa Library Quantification kit.
Video
Reliable library yield, even from low DNA input
Libraries generated from 300 ng, 75 ng, and 37.5 ng DNA all reach usable concentrations, demonstrating reliable library yield even as input decreases.
Low DNA input has usable library yield. Twist PCR-Free WGS Library Prep Kit libraries with Full Length UDI Adapters for PCR-free WGS were prepared using NA12878 300 ng, 75 ng, and 37.5 ng genomic DNA . Fragmentation parameters were set at 15 minutes and 25°C. Libraries were quantified by qPCR using the Kapa Library Quantification kit.
Library diversity stays high across DNA input levels
Estimated library size remains high from 300 ng down to 37.5 ng DNA input, indicating maintained library diversity and strong performance even with lower starting material.
Library diversity is maintained even with low DNA input. Twist PCR-Free WGS Library Preparation Kit libraries with Full Length UDI Adapters for PCR-free WGS were prepared using NA12878 300 ng, 75 ng, and 37.5 ng genomic DNA . Fragmentation parameters were set at 15 minutes and 25°C. Libraries were quantified by qPCR using the Kapa Library Quantification kit.
1 / 5
Reliable library yield, even from low DNA input
Libraries generated from 300 ng, 75 ng, and 37.5 ng DNA all reach usable concentrations, demonstrating reliable library yield even as input decreases.
Library diversity stays high across DNA input levels
Estimated library size remains high from 300 ng down to 37.5 ng DNA input, indicating maintained library diversity and strong performance even with lower starting material.
Which adapter system is right for you?
Twist's Adapter Systems improve the performance of any 'T-A' overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. They reduce adapter dimer formation, increase yield, and can eliminate or minimize PCR, improving data quality while lowering sequencing costs. With broad compatibility, high multiplexing (up to 3,072 indexes), and ready-to-use formats, they scale effortlessly from discovery to high-throughput studies. Quality is in our DNA—All Twist adapters are functionally validated to ensure performance and reliability in all NGS applications.
Features
Full Length UDI Adapters
UMI Adapter System
Universal Adapter System
HT Universal Adapter System
Adapter System - Trinity Freestyle
Best for
PCR-free Whole Genome Sequencing
Improved NGS variant accuracy
Most applications
High throughput applications
Used with the Trinity Fast Hyb Workflow
Contains
Full Length UDI Adapters
UMI Adapers; UDI Primers
Universal Adapters; UDI Primers
Universal Adapters; HT UDI Primers
Universal Adapters compatible with Trinity Freestyle Sequencing; UDI Primers
Unique indexes
1536
16 or 384
16 or 384
3072
384
Index length
10bp
10bp
10bp
12bp
10bp
Available formats
96-well plates
96-well plates
96-well plates
96- or 384-well plates
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Element Trinity Freestyle
Stay updated
Full Length UDI Adapters
Best for
PCR-free Whole Genome Sequencing
Contains
Full Length UDI Adapters
Unique indexes
1536
Index length
10bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
UMI Adapter System
Best for
Improved NGS variant accuracy
Contains
UMI Adapers; UDI Primers
Unique indexes
16 or 384
Index length
10bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Universal Adapter System
Best for
Improved NGS variant accuracy
Contains
UMI Adapers; UDI Primers
Unique indexes
16 or 384
Index length
10bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
HT Universal Adapter System
Best for
Most applications
Contains
Universal Adapters; UDI Primers
Unique indexes
16 or 384
Index length
10bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Adapter System - Trinity Freestyle
Best for
High throughput applications
Contains
Universal Adapters; HT UDI Primers
Unique indexes
3072
Index length
12bp
Available formats
96- or 384-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Intended Use: For Research Use Only. Not for use in diagnostic procedures.
Performance Disclaimer: Data shown derived from internal testing using NA12878 genomic DNA (300 ng, 75 ng, 37.5 ng) and Kapa® qPCR quantification. Individual library yield, concentration, and diversity results may vary depending on sample quality, input mass, operator technique, and platform instrumentation.
Trademarks: All third-party trademarks, trade names, and product names referenced herein are the property of their respective owners. Illumina® is a registered trademark of Illumina, Inc. Kapa® is a registered trademark of Roche. Element Cloudbreak® and Trinity™ are trademarks of Element Biosciences, Inc. Reference to third-party products or services does not imply endorsement, affiliation, or sponsorship by Twist Bioscience or trademark owners.
