The proprietary, Illumina-compatible design of Twist Universal Adapters engineered for broad compatibility with all ‘T-A’ overhang workflows and eliminates the formation of undesired adapter dimers. It also increases the workflow’s overall yield, which is especially beneficial before target enrichment. Twist Universal Adapter System (10 bp) Twist’s unique dual index (UDI) primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing. Twist HT Universal Adapter System (12 bp) Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high-throughput (HT) sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Minimize the formation of adapter dimers for higher data quality and improved sequencing efficiency.
Reduce the number of required PCR cycles via high ligation efficiency, for faster workflows and fewer amplification-induced errors.
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Support cost-effective studies with UDIs and improved sample demultiplexing on all high-throughput sequencing platforms.
Repeatable performance ensures confidence in results. When experimental conditions are held constant, Twist Universal Adapters, combined with the Twist TrueAmp Library Prep Kit, produces a robust, consistent DNA library fragment size across a wide range of sample inputs.
Post-amplification library concentration across a range of DNA inputs highlights sufficient yield for downstream applications even when starting with as little as 1 ng.
Repeatable performance ensures confidence in results. When experimental conditions are held constant, Twist Universal Adapters, combined with the Twist TrueAmp Library Prep Kit, produces a robust, consistent DNA library fragment size across a wide range of sample inputs.
Electropherograms of NGS libraries generated with the Twist TrueAmp Library Prep Kit. The overlap of the fluorescent curves demonstrates the consistency in library preparation that can be achieved from run to run. Samples were analyzed using the Agilent TapeStation D5000. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng were fragmented at 32°C. 3, 5, 6, 8, and 10 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates for each DNA input concentration.
Post-amplification library concentration across a range of DNA inputs highlights sufficient yield for downstream applications even when starting with as little as 1 ng.
Concentration of libraries after amplification for various DNA inputs. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng were fragmented at 32°C. 3, 5, 6, 8, and 10 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates for each DNA input concentration.
Repeatable performance ensures confidence in results. When experimental conditions are held constant, Twist Universal Adapters, combined with the Twist TrueAmp Library Prep Kit, produces a robust, consistent DNA library fragment size across a wide range of sample inputs.
Post-amplification library concentration across a range of DNA inputs highlights sufficient yield for downstream applications even when starting with as little as 1 ng.
Twist's Adapter Systems improve the performance of any 'T-A' overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. They reduce adapter dimer formation, increase yield, and can eliminate or minimize PCR—improving data quality while lowering sequencing costs. With broad compatibility, high multiplexing (up to 3,072 indexes), and ready-to-use formats, they scale effortlessly from discovery to high-throughput studies. Quality is in our DNA—All Twist adapters are functionally validated to support performance and reliability in all NGS applications.
Universal Adapter System
HT Universal Adapter System
Adapter System - Trinity Freestyle
Full Length UDI Adapters
UMI Adapter System
Best for
Most applications
High Throughput applications
Used with the Trinity Fast Hyb workflow
PCR-free Whole Genome Sequencing
Improved NGS variant accuracy
Contains
Universal Adapters; UDI Primers
Universal Adapters; HT UDI Primers
Universal Adapters compatible with Trinity Freestyle Sequencing; UDI Primers
Full Length UDI Adapters
UMI Adapers; UDI Primers
Unique Indexes
16 or 384
3072
384
1536
16 or 384
Index Length
10bp
12bp
10bp
10bp
10bp
Available Formats
96-well plates
96- or 384-well plates
96-well plates
96-well plates
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Element Trinity Freestyle
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Stay updated
Best for
Most applications
Contains
Universal Adapters; UDI Primers
Unique Indexes
16 or 384
Index Length
10bp
Available Formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Best for
High Throughput applications
Contains
Universal Adapters; HT UDI Primers
Unique Indexes
3072
Index Length
12bp
Available Formats
96- or 384-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Best for
High Throughput applications
Contains
Universal Adapters; HT UDI Primers
Unique Indexes
3072
Index Length
12bp
Available Formats
96- or 384-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Best for
Used with the Trinity Fast Hyb workflow
Contains
Universal Adapters compatible with Trinity Freestyle Sequencing; UDI Primers
Unique Indexes
384
Index Length
10bp
Available Formats
96-well plates
Sequencer Compatibility
Element Trinity Freestyle
Best for
PCR-free Whole Genome Sequencing
Contains
Full Length UDI Adapters
Unique Indexes
1536
Index Length
10bp
Available Formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Twist Universal Adapter Systems
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Trademarks: All trademarks, service marks, and company names are the property of their respective owners. Illumina®, TruSeq®, and Cloudbreak® are trademarks or registered trademarks of Illumina, Inc. TapeStation® is a registered trademark of Agilent Technologies, Inc. Reference to third-party products or services does not constitute or imply endorsement, sponsorship, or recommendation.
Intended Use: For Research Use Only. Not for use in diagnostic procedures.
Performance Disclaimer: Performance data and electropherogram results shown were obtained under specific experimental conditions. Individual results may vary depending on input quality, protocol execution, and instrumentation.
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