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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Twist Oligo Pool Amplification Guidelines
Recommended reagents and protocol for PCR amplification of Twist oligo pools.
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Twist uses phosphoramidite chemistry for oligo synthesis. Compared to conventional column-based synthesizers, our synthesis platform miniaturizes the synthesis process, which reduces reaction volumes and increases throughput. Because the resulting oligos are single-stranded and present at femtomole levels, they may need to be converted to double-stranded DNA or amplified for certain applications via PCR. PCR amplification also enriches the amounts of full-length oligo sequences.
