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Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability. We offer both Gene Fragments and Clonal Genes for your research needs.

Gene Fragments are linear synthesized DNA sequences for direct cloning or larger gene assembly. Clonal Genes are synthesized DNA cloned into a plasmid vector and NGS sequence verified.

Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.

Your Genes, Your Way

NGS verified, clonal-perfect genes
Delivered in your vector or Twist Catalog Vector
No Type II S sequence restrictions
High quality custom vector onboarding and verification

Industry Leading Price and Rapid Turnaround Time

From 9¢ per bp
11 to 17 business days

Intelligent Gene Synthesis

AI informed oligo design maximises success
>99% genes analysed accepted for synthesis

Scalable synthesis

No order limits
Order 1 gene or 10000 genes
No difference in turn around time

GENE SYNTHESIS NOW UP TO 5KB

NGS verified genes up to 5kb cloned into your vector or a catalog vector

No difference in turn around time

Synthesize large proteins and entire synthetic pathways

HIGHLY COMPATIBLE

5’ and 3’ Adapters as standard
Amplify all Gene Fragments with a single primer pair
Compatible with most cloning methods

Testimonials

We at Clariant onboarded a variety of our internal vectors for various host organisms at Twist Bioscience. We make use of Twist Bioscience's high success rate and fast turnover times of gene synthesis and custom cloning. Our goal is to outsource most of our routine molecular biology work to focus our research force and speed up our development times.

Dr. HELGE JOCHENS

Clariant

The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies.

DR. AMANDA PORTILLO

Institute of Systems and Synthetic Biology

This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.

DR. PHILIPPE MARLIERE

Institute of Systems and Synthetic Biology

What Scientists Have to Say

“

The combination of price and service allows us to do science that we could not otherwise do.

Professor Nicholas Harmer

Associate Professor • University of Exeter

“

The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies.

Dr. Amanda Portillo

Project Head • FluorAbodies • Sandia Biotech, Inc

“

...This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.

Dr. Philippe Marliere

Research Director • Institute of Systems & Synthetic Biology

Clonal Genes

Intelligent, reliable synthesis [missing new title]

Twist Bioscience’s platform is capable of synthesizing thousands of genes each month to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high-quality DNA synthesis and assembly; qualities that allow Twist to deliver perfect genes of various lengths and difficulty on-time when you need them.

The graph (left) represents Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.
The figure (right) is a graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is illustrated in green and no SNPs or indels were detected.

Gene Fragments

Reduce costs with high quality Gene Fragments

Accurate fragment synthesis accelerates discovery. Twist Bioscience gene fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.
Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene

Success Rate

The graph represents Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.

Clonal Genes

Size

0.3 - 5.0 kb

Vector Options

Twist Vector or vector of your choice

Price

From 9¢ per bp

Custom Cloning

From $25 per construct

Minimum Order

1 gene

Yield

50 ng - 2 ug

Turnaround Time

11 to 17 business days

Gene Fragments

Fragment Length

300-1,800 bp

Delivery format

Lyophilized dsDNA

Error rate

1:3000

Yield

100 ng - 1 μg per fragment

Order Minimum

1 fragment

Turnaround Time

6-9 business days

Build a better DNA Assembly Line
Arzeda leverages technology from Labcyte, TeselaGen and Twist Bioscience to accelerate their synthetic biology workflow.

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Speeding Up Antibody Discovery for Infectious Disease
Tasked with an ambitious goal from DARPA to develop a rapid response to help medical workers fight viral diseases in the field, Vanderbilt University Medical Center has already reduced the time to develop antibodies significantly. High-throughput, synthetic genes from Twist Bioscience have allowed the lab to expedite this process.

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Amyris discusses Next-Generation Platforms for Strain Optimization Watch this webinar showing how Twist Genes are enabling this innovative company with their microbial engineering efforts.

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Clonal Genes Product Sheet

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Twist Vectors Product Sheet

Twist Bioscience synthesizes high-quality, NGS-verified custom genes at a cost and scale that are otherwise unavailable. For researchers wanting to replicate their synthetic genes or use them in expression studies, Twist provides the option of delivery in a diverse selection of cloning and expression vectors through a convenient online ordering platform.

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Gene Fragments Product Sheet
Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs.

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