Shipping and Storage

We deliver unamplified single stranded DNA (>0.2 fmole per oligo), pooled in a single tube as dried down product. Each oligo will match the sequence provided by customer exactly, with an estimated error rate of 1:3000 nt.

1. Unmodified oligos are stable. For long-term storage (up to one year), freeze DNA at -20° C. For even longer storage, freeze DNA at -80° C.

2. If the oligos are stored in solution, avoid repeated freeze-thaw cycles as this process can lead to physical degradation of the oligo.

3. If numerous experiments are planned, aliquot the sample and store at -20°C.

4. Careful handling is recommended to avoid possible contamination with nucleases or bacteria.

5. Twist DNA resuspension guidelines can be found here.

Twist Bioscience ships its DNA products dried down or resuspended in 2 mL microcentrifuge tubes, 96-well plates, or 384-well plates. Dried DNA is shipped at ambient temperature and resuspended DNA is shipped frozen.

Double-stranded DNA and single-stranded oligonucleotides are stable under most standard laboratory storage conditions. However, it is important to consider the following best practices to maintain the high quality of the DNA synthesized by Twist Bioscience.

• Twist recommends storing Dried DNA for the following durations based on storage condition.
  • Room Temperature for 3 months.
  • 4° C for 12 months.
  • -20° C for 24 months.
  • -80° C for long term storage.
• Resuspension Guidelines
  • For resuspension, briefly centrifuge the tube or plate before opening and resuspend in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
  • A concentration of at least 10 ng/μL is recommended for the stock dilution, but the optimal concentration will need to be determined based on your desired application.
  • Prepare aliquots of the stock dilution and separate working aliquots to limit chances of contamination and to reduce the number of freeze/thaw cycles. Use working aliquots as soon as possible after preparation and minimize exposure to high temperatures.
• Resuspended DNA
  • Resuspension is available for some products. Your DNA may arrive in one of the following buffers. We recommend briefly centrifuging the tube or plate before opening.
    • Elution buffer (2 mM Tris-Cl, pH 8.5)
    • TE buffer (10 mM Tris-Cl pH 8.0, 1 mM EDTA)
    • TE Buffer low EDTA (10 mM Tris-Cl pH 8.0, 0.1 mM EDTA)
    • Water       
      • We do not recommend long-term storage in water.
  • Twist recommends storing Resuspended DNA for the following durations based on storage condition.
    • Room Temperature for 3 months.
    • 4° C for 12 months.
    • -20° C for 24 months.
    • -80° C for long term storage.

We deliver unamplified single stranded DNA (>0.2 fmole per oligo), pooled in a single tube as dried down product. Each oligo will match the sequence provided by customer exactly, with an estimated error rate of 1:3000 nt.

1. Unmodified oligos are stable. For long-term storage (up to one year), freeze DNA at -20° C. For even longer storage, freeze DNA at -80° C.

2. If the oligos are stored in solution, avoid repeated freeze-thaw cycles as this process can lead to physical degradation of the oligo.

3. If numerous experiments are planned, aliquot the sample and store at -20°C.

4. Careful handling is recommended to avoid possible contamination with nucleases or bacteria.

5. Twist DNA resuspension guidelines can be found here.

Twist Bioscience ships its DNA products dried down or resuspended in 2 mL microcentrifuge tubes, 96-well plates, or 384-well plates. Dried DNA is shipped at ambient temperature and resuspended DNA is shipped frozen.

Double-stranded DNA and single-stranded oligonucleotides are stable under most standard laboratory storage conditions. However, it is important to consider the following best practices to maintain the high quality of the DNA synthesized by Twist Bioscience.

• Twist recommends storing Dried DNA for the following durations based on storage condition.
  • Room Temperature for 3 months.
  • 4° C for 12 months.
  • -20° C for 24 months.
  • -80° C for long term storage.
• Resuspension Guidelines
  • For resuspension, briefly centrifuge the tube or plate before opening and resuspend in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
  • A concentration of at least 10 ng/μL is recommended for the stock dilution, but the optimal concentration will need to be determined based on your desired application.
  • Prepare aliquots of the stock dilution and separate working aliquots to limit chances of contamination and to reduce the number of freeze/thaw cycles. Use working aliquots as soon as possible after preparation and minimize exposure to high temperatures.
• Resuspended DNA
  • Resuspension is available for some products. Your DNA may arrive in one of the following buffers. We recommend briefly centrifuging the tube or plate before opening.
    • Elution buffer (2 mM Tris-Cl, pH 8.5)
    • TE buffer (10 mM Tris-Cl pH 8.0, 1 mM EDTA)
    • TE Buffer low EDTA (10 mM Tris-Cl pH 8.0, 0.1 mM EDTA)
    • Water       
      • We do not recommend long-term storage in water.
  • Twist recommends storing Resuspended DNA for the following durations based on storage condition.
    • Room Temperature for 3 months.
    • 4° C for 12 months.
    • -20° C for 24 months.
    • -80° C for long term storage.