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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Built for confident enzymatic methylation sequencing
Twist EM-seq v2 Methylation Detection System enables accurate methylation analysis through a fully enzymatic workflow designed to preserve DNA integrity while delivering consistent library yields and high conversion efficiency. Optimized for low input and challenging samples, EM-Seq v2 supports robust methylation profiling without bisulfite induced DNA damage.
Consistent library yields across input ranges
EM-Seq v2 delivers usable library yields from DNA inputs ranging from 0.1 - 200ng, supporting flexibility in experimental design while reducing dropout at low input levels.
High conversion efficiency with enzymatic chemistry
Optimized enzymatic conversion maintains high unmethylated cytosine conversion rates, supporting accurate methylation calls without harsh chemical treatment.
Engineered for methylation target enrichment
Reagents are specifically engineered to support methylation sequencing, preserving library complexity and performance through downstream workflows.
Product data
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
Figure 1 The Twist Methylation Enhancer improves target enrichment quality and reduces methylation-specific off-target hybridization for Twist EM-Seq v2 libraries captured with the Twist Alliance Pan-Cancer panel, using either the Fast or Standard Hybridization v2 systems.
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
Figure 2. Twist EM-Seq v2 Methylation Detection System libraries captured with the Twist Alliance Pan-Caner panel using the Twist Standard Hybridization v2 system provide highly reproducible Picard target enrichment performance metrics across samples with highly variable methylation levels.
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
Figure 3. Highly sensitive methylation detection. Detection of methylation is possible across a wide range of methylation levels and targets.
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
Figure 4. Yield of libraries generated with Twist EM-seq v2 Methylation Detection System. Library yields are high even with low mass input.
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
Figure 5. Left Panel: Non-CPG Conversion Rate. Conversion rates with v2 are high even with low mass input. Right Panel: Called CPG Ratio. Conversion specificity with v2 is high even with low mass input.
Video
Engineered reagents preserve performance post-conversion
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
Figure 1 The Twist Methylation Enhancer improves target enrichment quality and reduces methylation-specific off-target hybridization for Twist EM-Seq v2 libraries captured with the Twist Alliance Pan-Cancer panel, using either the Fast or Standard Hybridization v2 systems.
Consistent Methylation Hybridization Capture Performance
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
Figure 2. Twist EM-Seq v2 Methylation Detection System libraries captured with the Twist Alliance Pan-Caner panel using the Twist Standard Hybridization v2 system provide highly reproducible Picard target enrichment performance metrics across samples with highly variable methylation levels.
Reproducible Methylation Profiling Across Targets
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
Figure 3. Highly sensitive methylation detection. Detection of methylation is possible across a wide range of methylation levels and targets.
Consistent library yield across DNA inputs
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
Figure 4. Yield of libraries generated with Twist EM-seq v2 Methylation Detection System. Library yields are high even with low mass input.
Confident Enzymatic Conversion for Accurate CpG Methylation Calling
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
Figure 5. Left Panel: Non-CPG Conversion Rate. Conversion rates with v2 are high even with low mass input. Right Panel: Called CPG Ratio. Conversion specificity with v2 is high even with low mass input.
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Engineered reagents preserve performance post-conversion
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
Consistent Methylation Hybridization Capture Performance
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
Reproducible Methylation Profiling Across Targets
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
Consistent library yield across DNA inputs
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
Confident Enzymatic Conversion for Accurate CpG Methylation Calling
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
Highlighted resources
Case study
GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits
Ebook
GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits
Ebook
GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits
Poster
GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits
Ebook
GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits
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Related workflows and applications
