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The Power of Silicon-based DNA Synthesis

Custom antibody products in CHO can be used to help relieve bottlenecks in times of capacity constraints. Let Twist Express Antibodies, CHO, be an extension of your lab to provide you with fast, high-quality, purified antibodies ready for screening. Twist Express Antibodies produced in ExpiCHO™ cells, are harvested and either filtered (Supernatant) or purified using Protein A. The antibodies are then transferred to a preferred shipping container, frozen, and delivered along with the Glycerol Stocks of the individual chains in perfect shape for any functional assay or screening application.


• High-quality antibodies produced from NGS sequence-verified Twist Express Clonal Genes 
• Ready to use and scale-up ready using included Glycerol Stock

Twist Express Antibodies can be easily ordered using our integrated eCommerce application with just the Variable (VH & VL) sequences. Please read through our Quick Start Guide for ordering tips. The antibodies can also be thawed using our handling guidelines (here)  for use in any functional or screening assays.

 

Workflow

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We've enhanced our antibody offering by leveraging Express Genes for reduced TAT, expanding cell-line offerings and add-ons including normalization, endotoxin testing, and aliquoting to meet your protein production and purification needs.

Performance Data

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Figure Description: COVID Panel Expression Levels. Expression Panel of 66 IgGs generated from a COVID Antibody Discovery program. Average yields are represented by the bar while standard deviation is represented by the error bars for approximately 4 replicates of 1 mL expression volumes.

 

Twist Express Antibodies, CHO Product Offering

CHO Product Offering
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Our team is ready to answer all your questions.

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The Power of Silicon-based DNA Synthesis

Custom antibody products in HEK293 can be used to help relieve bottlenecks in times of capacity constraints. Let Twist Express Antibodies, HEK293, be an extension of your lab to provide you with fast, high-quality, purified antibodies ready for screening. Twist Express Antibodies produced in Expi293™ cells, are harvested and either filtered (Supernatant) or purified using Protein A. The antibodies are then transferred to a preferred shipping container, frozen, and delivered along with the Glycerol Stocks of the individual chains in perfect shape for any functional assay or screening application.

• High-quality antibodies produced from NGS sequence-verified Twist Express Clonal Genes 
• Ready to use and scale-up ready using included Glycerol Stock

Twist Express Antibodies can be easily ordered using our integrated eCommerce application with just the Variable (VH & VL) sequences. Please read through our Quick Start Guide for ordering tips. The antibodies can also be thawed using our handling guidelines (here)  for use in any functional or screening assays. 

 

Workflow

timeline
×

We've enhanced our antibody offering by leveraging Express Genes for reduced TAT, expanding cell-line offerings and add-ons including normalization, endotoxin testing, and aliquoting to meet your protein production and purification needs.

 

Performance Data

data
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Figure Description: Following antibody production, antibodies were purified and yields were determined. Of the 518 antibodies produced, 482 passed size and purity QC tests-409 yielded more than 100 ug per antibody.

 

Twist Express Antibodies, 293 Product Offering

HEK293 Product Offering
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Our team is ready to answer all your questions.

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Already Using Twist Express Antibodies? Unlock Even More Power with Twist Screening & Characterization Services

For scientists and teams developing antibodies, Twist's end-to-end antibody production and characterization platform is the solution for CROs and in-house setups as it combines AI/ML-ready data integration, flexible project design, and trusted manufacturing precision; all through a single, expert partner.

Train Discovery Algorithms Without a Wet Lab

Twist creates high-quality, standardized datasets ready for direct use in AI and machine learning models. Every step, from gene synthesis to expression to assay readouts, happens in-house, so you can focus on designing the next breakthrough antibody without investing in equipment, lab, space, or specialized staff.

Why Twist?

  • One, End-to-End Trusted Partner: Customers rely on Twist for DNA, this same quality and reliability extend through to antibody production and characterization.
  • AI/ML Model and Data Integration: For assay specific characterization needs, we have in house service expertise that enables accurate assay setup, data analysis, and data delivery for non standard services.
  • No Internal CAPEX Required: Twist serves as your external R&D engine, giving you access to precision and expertise without the need to invest in internal equipment, lab space, or staffing.
  • Flexible Project Design at Any Scale: Choose from CHO and HEK IgG formats and flexible characterization assays to meet your specific goals; whether filling workflow gaps or scaling up large customizable projects. Every project is tailored to your exact needs.
  • Scientific Expertise: Work directly with Twist's antibody experts to design and troubleshoot non-standard workflows (guide setup, optimize screening, and interpret complex data).
  • Speed Without Compromise: Proprietary gene synthesis technology enables rapid production of sequence-perfect antibodies, while automated screening ensures fast, reproducible characterization with minimal sample input.
  • Scalable from One to Thousands: Whether you're running a few candidates or high-throughput campaigns, Twist's infrastructure supports consistent turnaround and performance at any volume.

Explore our catalog of standard characterization assays available with:

  • Competitive turnaround times
  • Cost-effective pricing
  • Reliable reproducibility at scale
  • Low sample consumption

 

 

Category
Assay
Material Required (µg)
General Developability & Manufacturability
 aSEC 10
Tm, colloidal stability and size - nanoDSF + DLS 15
HIC (column-based) 10
Plate-based Hydrophobic Interaction (HI) 1
Intact mass (MS) 5
Self-Association
 CIC (column-based) 10
AC-SINS 20
Polyreactivity
 Plate-based Ovalbumin (OVA) Interaction 1
BVP-ELISA 10
Antigen binding interaction
 Carterra LSA - SPR - Affinity Ranking 20
Carterra LSA - SPR - Classical Epitope Bin 20
Octet BLI - Binding Check 10
Octet BLI - Affinity Ranking 10
Octet BLI - Epitope Bin 25

 

Don’t see what you’re looking for? Discover more with

 

For research use only, not for use in diagnostic procedures.

 

 

This document will provide you detailed information on Twist Bioscience’s Express Antibodies - CHO offering, including product specifications, customizations, and more.
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This document will provide you detailed information on Twist Bioscience’s Express Antibodies - HEK293 offering, including product specifications, customizations, and more
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Recommended handling and storage instructions for Twist High Throughput IgG
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This Quick start guide will walk you through the process of preparing your sequences and placing an order within Twist’s ordering platform.
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Twist's platform is designed to provide robust expression of antibody candidates with yields generated for potential antibody hits against a specific antigen. Learn about the expression data Twist will provide you in this flyer.
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Twist Express Antibodies: High-Throughput Custom Antibody Production
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Deep Repertoire Mining Uncovers Ultra-broad Coronavirus-Neutralizing Antibodies using Twist’s High Throughput Antibody Production and SPR Screening
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Ordering Process

To place an order through our eCommerce portal,simply prepare your trimmed variable region sequences according to our Quick start and paste them into our upload template along with the corresponding antibody names. Once the file is uploaded, the portal will ask for the IgG subtype, light chain type, expression vectors (Twist provided or custom), and to specify the Kozak and leader sequences to use. Once the sequences are screened and reviewed, simply select the scale (1 mL vs. 8 mL), grade (pure vs. supernatant), elution buffer, buffer exchange (optional), and delivery format to complete your order! 

 

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General

Twist supplies clonal gene glycerol stocks from the clonal genes synthesized for antibody production. During antibody production, the DNA is consumed for transfection; however, glycerol stocks are reserved and delivered along with the antibodies post-production. Please see out protocol for preparing fresh DNA from glycerol stocks. 

A leader or secretory signal sequence acts as a peptide signal bound by cellular machinery early in the translation process. This allows for the correct localization of the translation complex to the endoplasmic reticulum where the antibody chains can enter into the secretory pathway for protein assembly and secretion from the cell. The leader sequence is cleaved during the protein assembly process and is not a part of the final protein. Twist recommends the following leader sequence for both heavy and light chains when using our pTwist IgG expression vectors as these sequences have been tested and validated with our pTwist vectors in both Expi293™ and ExpiCHO™ cell lines:

VH: MRAWIFFLLCLAGRALA

VL: MRAWIFFLLCLAGRALA

The Kozak sequence directs the pre-initiation complex (PIC) and ribosome to the translation initiation site (start codon) and mediates ribosome assembly ensuring the correct protein sequence is translated.

The consensus Kozak sequence is generally considered as GCCGCCACCATGG, where ATG is the start codon (typically the start of the signal sequence). Twist recommends the version GCCGCCACC upstream of the start codon ATG.

You can use a Protein A or G solid phase to purify, which binds to the FC region of the antibodies, depending on species and isotype of IgG. Alternatively, you can use immobilized antigen (full length or short peptide antigen) to purify the antibodies. The method will depend on the downstream application and specificity of the antibody.

Twist uses the industry standard cell lines, Thermo Fisher Scientific Expi293™ and Thermo Fisher Scientific ExpiCHO™

Customers may select the pTwist CMV BG WPRE Neo expression vector when they would like to use their own Fc region sequence (IgG3, Mouse IgG, or modified Fc) or have another type of Fc-fusion antibody to express.

Please remember to include a stop codon at the end of your coding sequence. If you have any design questions, please email igg@twistbioscience.com for help with your custom request

Research has shown that C-terminal lysine residues of the heavy chain (present on all IgG isotypes) are cleaved off once the IgG is in circulation. Studies have also shown that there is no functional impact of the lysine once it’s cleaved and it’s presence is actually inhibitory to allowing the antibody to reach full cytotoxicity potential: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4622059/

 

This cystein in a full IgG forms a disulfide bond with the LC constant region which is not present in the VHH-Fc fusion construct.

Please ensure you have the following sequence format for uploading variable regions:

  • Removed signal leader peptide (if using pTwist vectors + Twist recommended signal leader peptide)
  • Variable regions should not end with any CH1 starting sequence
  • Sequences are divisible by three if your ORF is in line with your insertion site

You can find the pricing table for our custom antibody products in your account by clicking here.

If you need to contact your Account Manager regarding pricing, their contact information is available in eCommerce under Account.

    Delivery Format

We use commercially available PBS, please share catalog information with customer upon request:

  • PBS 7.4 Cat# 21-031-CM Corning
  • PBS 6.0 Cat# P1760 Teknova

Twist inspects every antibody product for yield (μg), purity (%), and size (kDa) under reducing conditions. Each antibody will be inspected via LabChip (CE-SDS) to inspect purity and size of the individual heavy and light chains. Yields are quantified using A280 readings. Supernatant samples are quantified using a titer determination method. In the finalized documentation, each purified antibody will have reported volume, yield, and concentration values in addition to percent purity and heavy chain and light chain sizing. Supernatant documentation will provide antibody titer expression levels of either low, medium or high expression levels.

1 mL = ~220 uL volume, 15-300 µg

8 mL = ~750 uL volume, 15-3000 µg

  • 96-deep well plates - custom plate maps available
  • 1.4 mL micronic tube racks
  • 2 mL tubes
  • 4 mL tubes
  • 10 mL tubes (for 8 mL supernatant only)

Resources and Guidance for Sequence Design

  1.  abYsis provides an integrated, comprehensive, and annotated sequence assessment for one or multiple antibodies. abYsis provides a thorough regional breakdown of antibody sequences to quickly identify where your variable region starts and ends. A tabular breakdown of the leader, FWK, and CDR sequences as well as the tail, or constant region sequence is also provided.

  2. It can be helpful to apply the annotations provided by abYsis to your sequence using bioinformatic programs to help visualize and isolate your variable region. Below is an example of a sequence imported to the Geneious Prime software platform with abYsis annotations applied.

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  1. One additional option that may provide comprehensive breakdown of    leader, variable, and constant regions is ANARCI. The resulting analysis provides the exact variable region sequence highlighted in red, which can then be uploaded to the Antibodies application on our eCommerce website. ANARCI also provides detail on the light chain domain type and the sequence species.

  2. Please keep in mind that variable region sequences should never start with a methionine (M). If you are uploading sequences that start with the amino acid M, or nucleotides ATG, and are planning to use pTwist vectors, please contact our support for assistance.

  3. Reformatting variable domains from one species to another can be complicated given differences between disulfide bonding and other features. By default, the Twist eCommerce platform will not correct any of these issues. We typically rely on you to account and inspect for these types of reformatting complications. If you need assistance, please contact our support team.

Tab testing 

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