Site Saturation Variant Libraries

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Generate 99% of Desired Variants

Protein engineering screens using single site variant libraries allow researchers to explore a protein’s sequence space and investigate the relationship between sequence and protein structure and function.

Twist Site Saturation Variant Library construction leverages massively parallel oligonucleotide synthesis using Twist's proprietary silicon-based DNA synthesis platform. Twist libraries are NGS-verified to confirm that all desired variants are present in the correct ratios.

Precisely Crafted Variant Libraries

Complete control over codon usage (all 64 codons available)
High uniformity of variant representation at every site
No unwanted codons or premature stop codons

Verified Quality

Rigorous quality control, including NGS verification of modified regions
Sequence variant ratios documented
Uniform distribution of user-defined variants
No premature stop codons or unwanted codons

Flexibility

Design all sequences, single or multiple domains and combinations— single, pairwise, or triple variants
Modular synthesis system enables iteration of future libraries
Greater flexibility than TRIM and the degenerate approach

Start Designing Your Library Now - A team of experts is waiting to help you design your perfect library for your specific needs. Order and Design here.

KRAS through large-scale saturation mutagenesis screens

Learn more about how Twist is helping drug developers characterize and catalog mutations in KRAS through large-scale saturation mutagenesis screens.

Experience base by base precision

Learn more about how Twist Single Site Variant Libraries (SSVL) enable the interrogation of sequence space to identify key residues in protein structure and function.

Did you know that tumor evolution is a significant problem for drug developers?

Learn how Twist is helping drug developers characterize and catalog mutations in KRAS through large-scale saturation mutagenesis screens.

Explore the Sequence Space

Site Saturation Libraries eliminate codon bias and unwanted substitutions unlike traditional methods such as degenerate and NNK approaches.

TRIM offers a poor repetitive yield, leading to <50% full-length product in a typical library whereas, Twist libraries yield more usable variants, increasing effective library size.

	ERROR PRONE PCR	DEGENERATE (NNK/NNS)	TWIST SITE SATURATION VARIANT LIBRARIES
Eliminates sequence bias	No	No	Yes
Number of codons available	Unknown	32	All 64
Prevents undesirable motifs	No	No	Yes
Allows codon optimization	No	No	Yes
Avoids stop codons	No	Yes	Yes

Highly Uniform Variant Libraries

Site Saturation Variant Libraries enable efficient sampling of a protein’s sequence space in screening assays.

This figure shows the observed distribution of amino acids across 65 positions in protein (19 intended variants per position) when built with Twist SSVLs versus error prone PCR. The bars represent a different amino acid position, with each color indicating the observed variant frequency. All variants are present in the expected ratios in the Twist Library.

A New Way of Designing Your Libraries

The New Library Design Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.

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Design Companion

Intuitive, interactive portal to easily upload your library sequence file
Identify potential errors in sequence with instant feedback enabling library validation on the fly
Save drafts for future reference

Real-time Optimization

Errors are flagged for review, letting you quickly resolve issues without delays
Our library design experts are still ready to assist you with any questions
With our intuitive tool and expert support we can help you find the fastest path to a validated, high-quality library

Faster Process

Real-time, interactive library design interface supports optimizing your library design and cost efficiently
Save drafts for future reference
Automatically generates a Statement of Work (SOW) for your order, expediting the entire ordering process

Expert Support Still at Your Fingertips

If you have any questions, please feel free to email us at library@twistbioscience.com

Take a peek at our Library Design Tool

A New Way of Designing Your Libraries at Twist

Watch Step by Step how to optimize your sequences

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Let’s get Started

The New Digital Library Design (DLD) Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.

Step 1

Log into your account. If you don’t have one, it only takes a few minutes to set it up!

Step 2

Start to design your library in the new interactive libraries design tool which has a step by step “how to” wizard. With this option you can optimize your library in real time with validations on the fly and real-time pricing and receive an SOW at the end of the submission process.