Where can I find a Certificate of Analysis (COA) for an oligo pool?

Certificates of Analysis (COA) are available for completed orders. You can find the COA for Oligo Pool products in your account by clicking here.


Still have questions? Contact Us

How should I resuspend my oligo pool?

Twist DNA products are dried down and shipped in either 96/384-well plates or 2 ml microcentrifuge tubes. Although double-stranded DNAs are stable under most standard storage conditions, we recommend the following to maintain high quality DNA:

  • Upon receipt, briefly centrifuge tube or plate and resuspend the DNA in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
  • We do not recommend resuspension in water.
  • A concentration of at least 10 ng/ul is recommended for a stock solution, but optimal concentration will need to be determined based on your desired application.
  • Prepare aliquots of the stock and separate working aliquots to limit the chances of contamination and reduce the number of freeze/thaw cycles.
  • For long term storage, freeze DNA at -20° C for up to one year, or at -80° C for even longer storage.

 

You can find more information here.  


Still have questions? Contact Us

What is the error rate of Twist's oligos?

Twist's oligos have an average error rate of about 1:3000 or less.  For more information, click here.  


Still have questions? Contact Us

What are Twist's oligo pools?

Twist's oligo pools are a user-designed collection of single stranded DNA oligonucleotides, ranging from 20 - 300 nt in length. The oligo pools are synthesized at high uniformity, with >90% of sequences present at signals within 2.5x of the mean, ensuring high uniformity and low dropout rate.

Additionally, industry-leading error rate of 1:3000 ensures the sequences that are ordered is what is delivered.


Still have questions? Contact Us

What are the guidelines for amplification of Twist's oligo pools?

Twist amplification guidelines for oligo pools can be found here.  Contact us at customersupport@twistbioscience.com if you have additional questions.


Still have questions? Contact Us

What concentration of DNA is delivered per oligo in an oligo pool?

Each oligo is present at > 0.2 fmol per oligo


Still have questions? Contact Us

Where can I find a Certificate of Analysis (COA) for an oligo pool?

Certificates of Analysis (COA) are available for completed orders. You can find the COA for Oligo Pool products in your account by clicking here.


Still have questions? Contact Us

How should I resuspend my oligo pool?

Twist DNA products are dried down and shipped in either 96/384-well plates or 2 ml microcentrifuge tubes. Although double-stranded DNAs are stable under most standard storage conditions, we recommend the following to maintain high quality DNA:

  • Upon receipt, briefly centrifuge tube or plate and resuspend the DNA in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
  • We do not recommend resuspension in water.
  • A concentration of at least 10 ng/ul is recommended for a stock solution, but optimal concentration will need to be determined based on your desired application.
  • Prepare aliquots of the stock and separate working aliquots to limit the chances of contamination and reduce the number of freeze/thaw cycles.
  • For long term storage, freeze DNA at -20° C for up to one year, or at -80° C for even longer storage.

 

You can find more information here.  


Still have questions? Contact Us

What is the error rate of Twist's oligos?

Twist's oligos have an average error rate of about 1:3000 or less.  For more information, click here.  


Still have questions? Contact Us

What are Twist's oligo pools?

Twist's oligo pools are a user-designed collection of single stranded DNA oligonucleotides, ranging from 20 - 300 nt in length. The oligo pools are synthesized at high uniformity, with >90% of sequences present at signals within 2.5x of the mean, ensuring high uniformity and low dropout rate.

Additionally, industry-leading error rate of 1:3000 ensures the sequences that are ordered is what is delivered.


Still have questions? Contact Us

What are the guidelines for amplification of Twist's oligo pools?

Twist amplification guidelines for oligo pools can be found here.  Contact us at customersupport@twistbioscience.com if you have additional questions.


Still have questions? Contact Us

What concentration of DNA is delivered per oligo in an oligo pool?

Each oligo is present at > 0.2 fmol per oligo


Still have questions? Contact Us