The Twist Methylation Target Enrichment with Fast Hybridization Protocol uses the Fast Hybridization Target Enrichment workflow to generate target-enriched DNA libraries for sequencing on Illumina next-generation sequencing (NGS) systems. This protocol details the steps for a high-throughput target enrichment system, designed to target specific custom sequences of interest using a hybridization time of 2-hours or less. A component of the Twist Targeted Methylation Sequencing solution, this protocol is:

• Designed for single or multiplex hybridization reactions using either Twist fixed or custom methylation panels; optional secondary methylation panels (spike-ins) can be added for additional content
• Optimized for use with the Twist-supplied NEBNext Enzymatic Methyl-seq v2 Library Preparation Kit

Download the protocol to learn more.