The Twist cfDNA Library Preparation Kit with the Twist UMI Adapter System is an optimized workflow for generating high-quality, indexed cfDNA libraries for Illumina NGS. Using reformulated enzymes, integrated UMI labeling, and Twist TrueAmp Polymerase, the protocol delivers robust performance from 1–20 ng of cfDNA, enabling accurate error correction, high library complexity, and reliable detection of low frequency variants. Designed for seamless compatibility with Twist target enrichment kits or WGS, the streamlined ~2.5-hour workflow supports scalable, automation-ready cfDNA sequencing with consistent, reproducible results.

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